The Genetic Manipulation of Staphylococci
Humana Press Inc. (Verlag)
978-1-4939-3157-6 (ISBN)
Practical and authoritative, The Genetic Manipulation of Staphylococci: Methods and Protocols serves as a vital resource and guide to scientists in the Staphylococcus community as they pursue their studies on these bacteria.
Understanding Staphylococcal Nomenclature.- Restriction-Modification Systems as a Barrier for Genetic Manipulation of Staphylococcus aureus.- Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci.- De Novo Assembly of Plasmids Using Yeast Recombinational Cloning.- Splicing by Overlap Extension PCR to Obtain Hybrid DNA Products.- Method for Preparation and Electroporation of S. aureus and S. epidermidis.- Rapid Isolation of DNA from Staphylococcus.- Bacteriophage Transduction in Staphylococcus aureus: Broth-Based Method.- Bacteriophage Transduction in Staphylococcus aureus.- Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus.- Conjugative Transfer in Staphylococcus aureus.- Allelic Exchange.- Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.- Generation of a Transposon Mutant Library in Staphylococcus aureus and Staphylococcus epidermidis Using Bursa aurealis.- Chemical and UV Mutagenesis.- Pulsed Field Gel Electrophoresis.- RNA-Sequencing of Staphylococcus aureus Messenger RNA.- Quantitative Real-Time PCR (qPCR) Workflow for Analyzing Staphylococcus aureus Gene Expression.- Electrophoretic Mobility Shift Assays.- Rapid Amplification of cDNA Ends for RNA transcript Sequencing in Staphylococcus.
Reihe/Serie | Methods in Molecular Biology ; 1373 |
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Zusatzinfo | 7 Illustrations, color; 16 Illustrations, black and white; X, 186 p. 23 illus., 7 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
Naturwissenschaften ► Biologie ► Allgemeines / Lexika | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Mutant generation • Nucleic Acid Analysis • pathogenic bacteria • Protein function • Single nucleotide changes • staphylococcus • Whole genome mutant libraries |
ISBN-10 | 1-4939-3157-1 / 1493931571 |
ISBN-13 | 978-1-4939-3157-6 / 9781493931576 |
Zustand | Neuware |
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