Baculovirus and Insect Cell Expression Protocols
Humana Press Inc. (Verlag)
978-1-4939-3042-5 (ISBN)
Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior.- Introduction to Baculovirus Molecular Biology.- Baculovirus Transfer Vectors.- Recombinant Baculovirus Isolation.- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System.- Available Lepidopteran Insect Cell Lines.- Lepidopteran Insect Cell Line Isolation from Insect Tissue.- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines.- Routine Maintenance And Storage Of Lepidopteran Insect Cell.- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System.- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System.- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors.- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae.- Production Of Virus-Like Particles For Vaccination.- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells.- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression.- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression.- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression.- Introduction To The Use Of Baculoviruses As Biological Insecticides.- Baculovirus Insecticide Production In Insect Larvae.- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses.- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants.- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production.- Gene Silencing In Insect Cells Using Rnai.- Using the Baculovirus/Insect Cell System to Study Apoptosis.- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.
Reihe/Serie | Methods in Molecular Biology ; 1350 |
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Zusatzinfo | 15 Illustrations, color; 50 Illustrations, black and white; XII, 508 p. 65 illus., 15 illus. in color. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | baculovirus molecular biology • BEVS • biopesticides • express genes • generating envelop-modified baculovirus • green fluorescent protein • insect cell genome • insect cell lines • RNAi • tubular reactors |
ISBN-10 | 1-4939-3042-7 / 1493930427 |
ISBN-13 | 978-1-4939-3042-5 / 9781493930425 |
Zustand | Neuware |
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