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Protocols for Neural Cell Culture -

Protocols for Neural Cell Culture

Buch | Softcover
388 Seiten
2001 | 3rd Revised edition
Humana Press Inc. (Verlag)
978-0-89603-902-5 (ISBN)
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The third edition of a collection of reproducible methods used in the neuroscience laboratory. The chapters carried over from the second edition have been extensively revised and updated, while 11 new chapters have been added.
Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 new chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as are essential procedures for hippocampal and microglial slice cultures. Protocols for Neural Cell Culture: Third Edition is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture and its disorders.

In Vitro Assays for Axonal Growth and Targeting Valerie Castellani and Jurgen Bolz Interface Organotypic Hippocampal Slice Cultures Dominique Muller, Nicolas Toni, Pierre-Alain Buchs, Lorena Parisi, and Luc Stoppini Slice Cultures for Study of Microglia Carol A. Colton, Meggan Czapiga, and Toby N. Behar Microexplant Cultures of the Cerebellum Bernard Rogister and Gustave Moonen Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons Robert B. Campenot and Grace Martin Chick Spinal Somatic Motoneurons in Culture Bernhard H. J. Juurlink Primary Cultures of Sympathetic Ganglia Mary I. Johnson Primary Cell Cultures for the Study of Myelination Mary I. Johnson, Richard P. Bunge, and Patrick M. Wood Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures Ruth Cole and Jean de Vellis Culture of Glial Cells from Human Brain Biopsies V. Wee Yong and Jack P. Antel Cultures of Astroglia and Microglia from Primary Cultures of Mouse Neopallium Sergey Fedoroff and Arleen Richardson Cultures of Oligodendroblasts from Primary Cultures of Rat Neopallium Bernhard H. J. Juurlink, Shawn K. Thorburne, and Richard M. Devon Olfactory Ensheathing Cell Cultures Ronald Doucette Cultures of Stem Cells of the Central Nervous System Angela Gritti, Rossella Galli, and Angelo Luigi Vescovi Aggregating Neural Cell Cultures Paul Honegger and Florianne Monnet-Tschudi Neural Cell Lines Kaoru Murayama, Natalia N. Singh, Angela Helmrich, and David W. Barnes Virus Vectors for Gene Therapy of the Nervous System Sonia N. Yeung and Frank Tufaro Preparation of Substrata for In Vitro Culture of Neurons Paul C. Letourneau Serum-Fee Media for Neural Cell Cultures: Adult and Embryonic Paul J. Price and Gregory J. Brewer Development of Multiple-Well Plate Biological Assays John W. Harbell Neural Cell Counting: Cell Number Determination in Microplate Cultures Marston Manthorpe Assays for Neuronal Cell Migration Toby N. Behar Colony Culture: Plating Efficiency Assay and Cloning Sergey Fedoroff and Arleen Richardson Hybridoma Cultures for Production of Antibodies: Purification and Specificity of Antibodies J. Denry Sato Elimination of Cell Types from Mixed Neural Cell Cultures Richard M. Devon Quantification of Cells in Culture Arleen Richardson and Sergey Fedoroff Tips for Tissue Culture Arleen Richardson and Sergey Fedoroff

Verlagsort Totowa, NJ
Sprache englisch
Maße 280 x 210 mm
Gewicht 871 g
Einbandart Kunststoff
Themenwelt Medizin / Pharmazie Studium
Naturwissenschaften Biologie Humanbiologie
Naturwissenschaften Biologie Zellbiologie
Naturwissenschaften Biologie Zoologie
ISBN-10 0-89603-902-1 / 0896039021
ISBN-13 978-0-89603-902-5 / 9780896039025
Zustand Neuware
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