Phage Display
Oxford University Press (Verlag)
978-0-19-963873-4 (ISBN)
This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensively cross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential reading for anyone currently using, or wishing to use the technique for basic research and drug discovery.
Dr Tim Clackson Vice President, Gene Therapy and Genomics ARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA +1 617 494 0400 ext. 258 +1 617 225 2589 clackson@ariad.com Tim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years in a variety of research and research management positions. His group is combining chemistry, cell biology and protein engineering to develop techniques for regulating gene expression and other intracellular processes using small molecules. Dr Clackson's particular research interest is the use of protein engineering and chemical biology techniques to analyze and redesign the interfaces between molecules, especially small molecule-protein interfaces. Dr Henry B. Lowman Senior Scientist Genentech Inc., 1 DNA Way, South San Francisco, CA 94080, USA +1 650 225 1171 +1 650 225 3734 hbl@gene.com Henry B. Lowman, Ph.D. is a Senior Scientist and Associate Director of Antibody Engineering with joint appointments in Immunology and Protein Engineering, at Genentech, Inc., where he has been engaged in research on proteins and peptides for over thirteen years.
1. Introduction to phage biology and phage display ; 2. Constructing phage display libraries by oligonucleotide-directed mutagenesis ; 3. In vitro DNA recombination ; 4. Phage selection strategies for improved affinity and specificity of proteins and peptides ; 5. Rapid screening of phage displayed protein binding affinities by phage ELISA ; 6. Identification of novel ligands for receptors using recombinant peptide libraries ; 7. Substrate phage display ; 8. Protease-based selection of stably folded proteins and protein domains from phage display libraries ; 9. Phage display of zinc fingers and other nucleic acid-binding motifs ; 10. In vivo and ex vivo selections using phage-displayed libraries ; 11. Screening phage libraries with sera ; 12. Interaction cloning using cDNA libraries displayed on phage ; 13. Phage antibody libraries ; 14. Affinity maturation of phage antibodies
| Erscheint lt. Verlag | 26.2.2004 |
|---|---|
| Reihe/Serie | Practical Approach Series ; 266 |
| Zusatzinfo | numerous figures, tables and one halftone |
| Verlagsort | Oxford |
| Sprache | englisch |
| Maße | 189 x 246 mm |
| Gewicht | 715 g |
| Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Pathologie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-19-963873-X / 019963873X |
| ISBN-13 | 978-0-19-963873-4 / 9780199638734 |
| Zustand | Neuware |
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