Introduction to Medical Laboratory Technology (eBook)

F. J. Baker, Eveline D. Luckcock, R. E. Silverton (Autoren)

eBook Download: PDF

2014 | 1. Auflage
342 Seiten
Elsevier Science (Verlag)
978-1-4831-9541-4 (ISBN)

Infos

An Introduction to Medical Laboratory Technology, Second Edition provides information pertinent to medical laboratory technology. This book discusses the importance of laboratory technology in hospital practice. Organized into seven sections encompassing 33 chapters, this edition begins with an overview of the role of the medical technologist in the diagnosis of disease by the use of certain accepted laboratory methods. This text then explains the general types of glassware that is widely used in medical laboratories. Other chapters consider the main methods of estimating the sugar content of body fluids, methods in feces and gastric analysis, and microscopical and chemical examination of urine. This book discusses as well the microscopic examination of bacteria, which necessitates making smears and hanging-drop preparations on microscope slides. The final chapter deals with some aspects of elementary physiology. This book is a valuable resource for students and junior technicians, as well as for qualified technologists and medical students.

Inhalt

Front Cover 1
An Introduction to Medical Laboratory Technology 8
Copyright Page 9
Table of Contents 14
FOREWORD 10
PREFACE TO THE SECOND EDITION 12
PREFACE TO THE FIRST EDITION 13
ACKNOWLEDGEMENTS 17
INTRODUCTION 18
SECTION I: GENERAL 20
CHAPTER 1. GENERAL LABORATORY GLASSWARE 22
CLEANING OF GLASSWARE 23
STANDARDIZED GLASSWARE 25
GENERAL GLASSWARE 26
CHAPTER 2. GENERAL LABORATORY APPARATUS 40
ACKNOWLEDGMENTS 57
CHAPTER 3. ELEMENTARY GLASS-BLOWING 58
ACKNOWLEDGMENTS 64
CHAPTER 4. ELEMENTARY MICROSCOPY 65
COMPONENTS OF THE MICROSCOPE 67
MAGNIFICATION 75
SETTING UP THE MICROSCOPE 75
THE BINOCULAR MICROSCOPE 76
SOME DO'S AND DON'TS OF MICROSCOPY 76
ACKNOWLEDGMENTS 77
SECTION II: CHEMISTRY 78
CHAPTER 5. GENERAL CHEMISTRY 79
VALENCY 81
FUNDAMENTAL LAWS OF CHEMISTRY 83
THE GAS LAWS 84
CHEMICAL EQUATIONS 87
EQUIVALENTS 88
ACIDS, BASES, ALKALIS AND SALTS 90
SOME MATHEMATICAL EXPRESSIONS 91
HYDROGEN ION CONCENTRATION (pH) 94
CHAPTER 6. ELEMENTARY COLORIMETRY 104
VISUAL COLORIMETERS 104
PHOTO-ELECTRIC ABSORPTIOMETERS 107
ACKNOWLEDGMENTS 109
CHAPTER 7. URINE 110
ROUTINE URINE EXAMINATION 111
FURTHER CHEMICAL INVESTIGATIONS 120
CHAPTER 8. BLOOD 128
BLOOD SUGAR 128
BLOOD UREA 133
CHAPTER 9. FAECES AND GASTRIC ANALYSIS 139
NORMAL AND ABNORMAL CONTENT 139
MICROSCOPICAL EXAMINATION 141
GASTRIC ANALYSIS 143
CHAPTER 10. EXAMINATION OF CEREBROSPINAL FLUID 147
SECTION II.: HISTOLOGY 154
CHAPTER 11. FIXATION 155
SIMPLE FIXATIVES 156
COMPOUND FIXATIVES 159
THE FIXATION OF GROSS SPECIMENS 164
'WASHING OUT' 165
CHAPTER 12. DECALCIFICATION 167
CHAPTER 13. PARAFFIN WAX TECHNIQUE 171
DEHYDRATION 171
CLEARING 173
IMPREGNATION WITH PARAFFIN WAX 174
AUTOMATIC TISSUE PROCESSERS 177
VACUUM EMBEDDING TECHNIQUE 178
ACKNOWLEDGMENTS 180
CHAPTER 14. SECTION CUTTING 181
THE MICROTOME 181
TECHNIQUE OF SECTION CUTTING 188
MOUNTING THE SECTION ON SLIDES 191
ACKNOWLEDGMENTS 192
CHAPTER 15. BIOLOGICAL STAINING 193
NATURAL DYES 193
SYNTHETIC DYES 195
BASIC, ACID AND NEUTRAL DYES 196
THE STAINING PROPERTIES OF DYES 196
CHAPTER 16. STAINING PROCEDURES 199
STAINING EQUIPMENT 199
PROCEDURE FOR STAINING PARAFFIN WAX SECTIONS 199
PIGMENTS 200
MOUNTING OF SECTIONS 202
FORMULAE OF STAINS AND REAGENTS 203
TECHNIQUES AND RESULTS 211
SECTION IV: BACTERIOLOGY 218
CHAPTER 17. INTRODUCTION TO BACTERIOLOGY 220
MORPHOLOGY OF BACTERIA 221
MICROSCOPIC EXAMINATION 224
CHAPTER 18. STERILIZATION 228
PHYSICAL AGENCIES 228
CHEMICAL AGENCIES 230
STERILIZATION BY FILTRATION 231
CHAPTER 19. THE USE OF CULTURE MEDIA 234
PLATE CULTURAL METHODS 236
TUBE CULTURAL METHODS 237
CHAPTER 20. PREPARATION OF CULTURE MEDIA 240
THE ADJUSTMENT OF pH 242
NOTES ON ESTIMATING THE pH OF MEDIA 244
PREPARATION OF CULTURE MEDIA 245
CARBOHYDRATE MEDIA 250
MEDIA FOR SPECIAL PURPOSES 253
MEDIA FOR ISOLATION OF CORYNEBACTERIUM GROUP 256
EGG MEDIUM 257
CHAPTER 21. ANAEROBIC METHODS 259
CHAPTER 22. SIMPLE AGGLUTINATION TEST 263
DILUTIONS OF SERUM FOR THE AGGLUTINATION TEST 265
CHAPTER 23. ROUTINE BACTERIOLOGICAL EXAMINATION OF SPECIMENS 266
BLOOD CULTURES 267
CEREBROSPINAL FLUID 268
FAECES 268
FLUIDS 269
PUS 270
SEROLOGY 270
SPUTUM 271
SWABS 273
GASTRIC LAVAGE 274
URINE 275
SENSITIVITY TESTS 276
POSTAGE OF PATHOLOGICAL SPECIMENS 277
CHAPTER 24. THE CARE AND MAINTENANCE OF LABORATORY ANIMALS 278
EUTHANASIA 286
PREPARATION OF ANIMALS FOR POST-MORTEM 287
ACKNOWLEDGEMENT 287
SECTION V: HAEMATOLOGY 288
CHAPTER 25. INTRODUCTION TO HAEMATOLOGY 289
STRUCTURE AND FUNCTION OF BLOOD CELLS 289
BLOOD COAGULATION 293
BLOOD GROUPS 294
CHAPTER 26. HAEMATOLOGICAL TECHNIQUES 296
COLLECTION OF BLOOD 296
BLOOD COUNTS 297
RED CELL COUNTING METHODS 302
WHITE CELL COUNTING METHODS 306
SOURCES OF ERROR IN COUNTING 307
DIFFERENTIAL LEUCOCYTE COUNT 308
HAEMOGLOBIN ESTIMATION 309
SEDIMENTATION RATE (E.S.R. or B.S.R.) 312
PACKED CELL VOLUME (P.C.V.) 313
RED CELL FRAGILITY TEST 314
RETICULOCYTE COUNT 315
CHAPTER 27. THE COLOUR INDEX AND ABSOLUTE VALUES 317
SECTION VI: BLOOD TRANSFUSION TECHNIQUE 320
CHAPTER 28. INTRODUCTION TO BLOOD TRANSFUSION TECHNIQUES 321
CHAPTER 29. BLOOD GROUPS 327
ABO GROUPING SYSTEM 327
OTHER BLOOD-GROUP SYSTEMS 331
COMPATIBILITY TESTS 331
CHAPTER 30. BLOOD GROUPING TECHNIQUES 333
ABO GROUPING 333
RHESUS GROUPING 339
CHAPTER 31. SOLUTIONS AND APPARATUS USED INBLOOD TRANSFUSION 342
ANTICOAGULANT SOLUTION 342
COPPER SULPHATE SOLUTION FOR HAEMOGLOBIN ESTIMATION 343
TAKING AND GIVING SETS (Fig. 110) 343
CHAPTER 32. BLOOD STORAGE AND REFRIGERATION PRINCIPLES 347
STORAGE OF BLOOD 347
STORAGE OF SERA 348
STORAGE OF ALBUMIN 348
PRINCIPLES OF REFRIGERATION 348
CARBON DIOXIDE REFRIGERATION 351
REFRIGERATION AND THE TRANSPORT OF BLOOD 352
SECTION VII: PHYSIOLOGY 354
CHAPTER 33. SOME ASPECTS OF ELEMENTARY PHYSIOLOGY 355
INTRODUCTION 355
DIGESTIVE SYSTEM 356
THE LIVER 361
THE URINARY SYSTEM 362
SKIN 364
BLOOD 364
RESPIRATION 366
BOOKS CONSULTED AND RECOMMENDED FOR FURTHER READING 383
GLOSSARY 384
NOTES 397
INDEX 402

Produkt-Details

• Erscheint lt. Verlag: 12.5.2014
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete
• Themenwelt: Studium ► 2. Studienabschnitt (Klinik) ► Anamnese / Körperliche Untersuchung
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• Themenwelt: Naturwissenschaften ► Biologie ► Biochemie
• Themenwelt: Technik ► Bauwesen
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• ISBN-10: 1-4831-9541-4 / 1483195414
• ISBN-13: 978-1-4831-9541-4 / 9781483195414