Manual of Immunopathological Techniques
Jaypee Brothers Medical Publishers (Verlag)
978-93-5152-084-9 (ISBN)
The use of immunopathological techniques has become increasingly widespread due to their ability to not only diagnose infectious and immunodeficiency disorders, but also haematological, oncological and gastroenterological diseases.
This manual is a practical guide to immunopathological techniques for undergraduates and trainees. Beginning with an overview of immunopathology, the following chapters provide in depth discussion on different techniques for the diagnosis and management of numerous immunological disorders, including autoimmune diseases, tumours, transplants and immunohistochemistry.
Presented in bullet point format for easy reference, this useful text is enhanced by high quality photographs and tables to assist learning.
Key points
Practical guide to immunopathological techniques
Covers different techniques for diagnosis and treatment of numerous disorders
Presented in bullet point format for easy reference
Highly illustrated with clinical photographs and tables
Usha MD Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India Gyanendra Kumar Sonkar PhD Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India Sangeeta Singh PhD Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
Introduction
Tests for cell mediated immunity (CMI)
Lymphocytes typing by Flow Cytometry
Diagnosis of Kala-Azar by PCR technique
Test for myeloma profile and qualitative assessment of serum proteins
Agarose gel electrophoresis for qualitative assessment of serum proteins
Cellulose Acetate Electrophoresis
Agar Gel Immunoelectrophoresis for typing of myeloma
Micro Agar Gel Double Diffusion Technique
Counter Current Immunoelectrophoresis
Single Radial Immunodiffusion and Immunoturbidometric methods for the estimation of Immunoglobulins
Estimation of complement C3, C4 and hemolytic assay for serum complement
Demonstration of immune complexes and complement by indirect immuno peroxidase method
Test for autoimmune diseases
Test for Autoantibodies by ELISA technique
Acid Fuchsin Orange G in kidney biopsy for demonstration of immune complexes
Detection of tumor markers in paraffin embedded sections by immunohistochemistry
Detection of circulating immune complexes (CIC) in the serum
Demonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescence
HLA typing and Cross Match test in solid organ transplantation
HLA B27 typing by PCR method
Fluorescence in-situ hybridisation: methods and applications
Detection of cytokines (IL-6, IL-8, TNF-a)
Western Blot or Tests for detection of antibody to extractable nuclear antigen
Detection of anti-thyroglobulin antibodies by hemagglutination method
Distillation and demineralization of water
Raising of polyclonal anti human anti-IgG in rabbit
Erscheint lt. Verlag | 30.11.2014 |
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Zusatzinfo | 19 Tables, unspecified; 31 Halftones, color; 5 Illustrations, unspecified |
Verlagsort | New Delhi |
Sprache | englisch |
Maße | 171 x 241 mm |
Gewicht | 290 g |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Pathologie |
ISBN-10 | 93-5152-084-6 / 9351520846 |
ISBN-13 | 978-93-5152-084-9 / 9789351520849 |
Zustand | Neuware |
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