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Sepsis

Diagnostic Methods and Protocols

Nicasio Mancini (Herausgeber)

Buch | Hardcover

262 Seiten

2014
Humana Press Inc. (Verlag)
978-1-4939-1775-4 (ISBN)

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This volume covers microbiological, clinical and patophysiological aspects of sepsis and also provides general overview chapters with every chapter discussing the real clinical impact of the discussed diagnostic approaches. Sepsis is a major clinical problem that takes an inordinate toll on human lives and economical resources. It is widely recognized that inappropriate treatment is associated with a dramatic increase in mortality, especially within the first hours, therefore clinical and microbiological diagnosis are of pivotal importance in the management of septic patients. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Sepsis: Diagnostic Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this life-threatening illness.

Microbiological Diagnosis of Sepsis: The Confounding Effect of a "Gold Standard".- Pathopysiological Aspects of Sepsis: An Overview.- Clinical Aspects of Sepsis: An Overview.- Technical Improvements in Culturing Blood.- Matrix-Assisted Laser-Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF/MS)-Based Identification of Pathogens from Positive Blood Culture Bottles.- Bacterial and Fungal DNA Extraction from Positive Blood Culture Bottles: A Manual and an Automated Protocol.- Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Sequencing Approach.- Identification of Bacterial and Fungal Pathogens from Positive Blood Culture Bottles: A Microarray-Based Approach.- Detection of Carbapenemases Using Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay.- Molecular Detection of Antibiotic Resistance Genes from Positive Blood Cultures.- Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols.- Bacterial and Fungal DNA Extraction from Blood Samples: Automated Protocols.- Broad-Range-PCR in the Identification of Bacterial and Fungal Pathogens from Blood Samples: A Sequencing Approach.- Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples.- Host Response Biomarkers in the Diagnosis of Sepsis: A General Overview.- Host Response Biomarkers in Sepsis: The Role of Procalcitonin.- Host Response Biomarkers in Sepsis: Overview on sTREM-1 Detection.- Host Response Biomarkers in Sepsis: suPAR Detection.- Clinical Diagnosis of Sepsis and the Combined Use of Biomarkers, Culture- and Non-Culture-Based Assays.

Reihe/Serie	Methods in Molecular Biology ; 1237
Zusatzinfo	18 Illustrations, color; 9 Illustrations, black and white; XIII, 262 p. 27 illus., 18 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Pathologie
	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Biomarkers • blood culture • broad-range PCR • fungal DNA • Pathogens
ISBN-10	1-4939-1775-7 / 1493917757
ISBN-13	978-1-4939-1775-4 / 9781493917754
Zustand	Neuware