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Regulatory Non-Coding RNAs

Methods and Protocols

Gordon G. Carmichael (Herausgeber)

Buch | Hardcover

181 Seiten

2014
Humana Press Inc. (Verlag)
978-1-4939-1368-8 (ISBN)

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Regulatory Non-Coding RNAs: Methods and Protocols offers a collection of methods for those interested in the discovery, localization, and functional analysis of these non-coding transcripts that have the potential and ability to orchestrate and control gene expression. After a review of the field, this detailed volume continues with methods useful for the study of siRNAs, microRNAs and their targets, techniques concerned with long non-coding RNAs, as well as studies of the critical parameters of functional non-coding RNA protein-RNA interactions and the environment in which they act. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips for troubleshooting and avoiding known pitfalls.

Dependable and easy to use, Regulatory Non-Coding RNAs: Methods and Protocols provides a current, state-of-the-art collection of methods and approaches that will be of value to researchers in this expanding and fascinating field.

Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs.- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.- A Biochemical Approach to Identify Direct MicroRNA Targets.- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs.- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets.- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells.- Affinity-Purification of Long Noncoding RNA−Protein Complexes from Formaldehyde-Cross linked Mammalian Cells.- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins.- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale.- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization.- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells.- Locating RNAs In Situ with FISH-STIC Probes.- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides.- On Using Magnesium and Potassium Ions in RNA Experiments.- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.

Reihe/Serie	Methods in Molecular Biology ; 1206
Zusatzinfo	10 Illustrations, color; 29 Illustrations, black and white; X, 181 p. 39 illus., 10 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete
Themenwelt	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
Schlagworte	Fluorescence in situ hybridization • long non-coding RNAs • MicroRNA targets • Non-coding transcriptome • Protein-RNA interactions • RNA-binding proteins • siRNAs
ISBN-10	1-4939-1368-9 / 1493913689
ISBN-13	978-1-4939-1368-8 / 9781493913688
Zustand	Neuware