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Enzyme Assays -

Enzyme Assays

Buch | Softcover
302 Seiten
2002 | 2nd Revised edition
Oxford University Press (Verlag)
978-0-19-963820-8 (ISBN)
CHF 159,95 inkl. MwSt
Enzyme assays are among the most frequently performed procedures in biochemistry, routinely used (for example) to estimate the amount of enzyme present in a cell or tissue. This new edition describes the design and execution of enzyme assays, covering both general principles and specific examples.
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.

Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration.

This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

1. Principles of enzyme assay and kinetic studies ; 2. Photometric assays ; 3. Radiometric assays ; 4. High performance liquid chromatography assays ; 5. Electrochemical assays: the oxygen electrode ; 6. Electrochemical assays: the nitric oxide electrode ; 7. Electrochemical assays: the pH-stat ; 8. Enzyme assays after gel electrophoresis ; 9. Techniques for enzyme extraction ; 10. Determination of active site concentration ; 11. High throughput screening - considerations for enzyme assays ; 12. Statistical analysis of enzyme kinetic data

Erscheint lt. Verlag 4.4.2002
Reihe/Serie Practical Approach Series ; 257
Zusatzinfo 2 halftones, numerous figures
Verlagsort Oxford
Sprache englisch
Maße 191 x 247 mm
Gewicht 594 g
Themenwelt Medizin / Pharmazie Medizinische Fachgebiete Pharmakologie / Pharmakotherapie
Studium 1. Studienabschnitt (Vorklinik) Physiologie
Naturwissenschaften Biologie Biochemie
Naturwissenschaften Chemie Analytische Chemie
ISBN-10 0-19-963820-9 / 0199638209
ISBN-13 978-0-19-963820-8 / 9780199638208
Zustand Neuware
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