Basic Methods In Molecular Biology
Appleton & Lange (Verlag)
978-0-8385-0642-4 (ISBN)
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General methods; bacterial strains and cloning vectors; enzymes that modify DNA and RNA; in vitro amplification of DNA using - the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase, introduction; DNA restriction fragment analysis and preparation, introduction; in vitro labeling of probes and filter hybridization; plasmid DNA preparation for E. Coli hosts; preparation of DNA from Lambda bacteriophage clones; subcloning fragments into plasmid vectors and plasmid construction; preparation of genomic DNA; preparation and analysis of RNA from eukaryotic cells - overview; geonomic cloning; generatino of cDNA libaries; preparation of subtractive cDNA, introduction; chain termination sequencing; transfection of mammalian cells, introduction; basic methods of protein analysis; in situ hybridization; transgenic mouse preparation; detection and in vitro generation of specific mutations in genes and cDNAs, introduction; appendices.
| Erscheint lt. Verlag | 31.3.1994 |
|---|---|
| Verlagsort | Maidenhead |
| Sprache | englisch |
| Maße | 193 x 259 mm |
| Gewicht | 1233 g |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| ISBN-10 | 0-8385-0642-9 / 0838506429 |
| ISBN-13 | 978-0-8385-0642-4 / 9780838506424 |
| Zustand | Neuware |
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