Molecular Histochemical Techniques

Buch | Softcover

263 Seiten

2012
Springer Verlag, Japan
978-4-431-68520-3 (ISBN)

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Molecular Histochemical Techniques presents detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. In situ nick translation and TUNEL are described in detail, along with radioactive and nonradioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, a new development in molecular histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. Methodology is set forth step-by-step in easy-to-follow language by leading scientists who are working in the field today. The book serves as both a rich source of the latest information and a practical lab manual.

1 Introduction to the Detection of DNA Strand Breaks.- 2 In Situ Nick Translation.- 3 In Situ Nick End-Labeling: Light Microscopical.- 4 In Situ Nick End-Labeling: Electron Microscopical.- 5 Introduction to the Detection of Specific DNA and RNA Sequences.- 6 In Situ Hybridization for DNA: Fluorescent Probe.- 7 In Situ Hybridization for RNA: Radioactive DNA Probe.- 8 In Situ Hybridization for RNA: Radioactive RNA Probe — A Protocol for Practice With a Kit.- 9 In Situ Hybridization for RNA: Nonradioactive probe: ds cDNA Probe.- 10 In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe.- 11 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer.- 12 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (I).- 13 In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II).- 14 In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe.- 15 Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistry.- 16 Whole Mount in Situ Hybridization for mRNA detection in Chick Embryos.- 17 Introduction to the Detection of Transcription Regulatory Proteins.- 18 Southwestern Histochemistry.

Reihe/Serie	Springer Lab Manuals
Zusatzinfo	X, 263 p.
Verlagsort	Tokyo
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Medizin / Pharmazie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	DNA strand breaks • In situ Hybridization
ISBN-10	4-431-68520-0 / 4431685200
ISBN-13	978-4-431-68520-3 / 9784431685203
Zustand	Neuware