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Nucleic Acid Electrophoresis -

Nucleic Acid Electrophoresis

Dietmar Tietz (Herausgeber)

Buch | Softcover
XIII, 328 Seiten
2012 | 1998
Springer Berlin (Verlag)
978-3-642-47763-8 (ISBN)
CHF 74,85 inkl. MwSt
Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.

1 DNA Gel Electrophoresis.- 2 Denaturating Electrophoresis in DNA Sequencing: A Brief History and Current Protocols.- 3 An Introduction to DNA Fingerprinting Using RFLP and RAPD Techniques.- 4 Pulsed-Field Gel Electrophoresis for Genomic Fingerprinting of Pathogenic Bacteria.- 5 Ultrasensitive Silver Based Stains for Nucleic Acid Detection.- 6 The Analysis of Microsatellites by Silver Staining of Nondenaturing Polyacrylamide Gels.- 7 Capillary Electrophoretic Separation of DNA Fragments.- 8 Rapid Detection of Hepatitis C Virus in Plasma and Liver Biopsies by Capillary Electrophoresis.- 9 Electrophoresis in the Field of Forensic Science.- 10 The Electrophoretic Mobility Shift Assay (EMSA) for Detection and Analysis of Protein-DNA Interactions.- 11 Mobility Shift Analysis of Protein-DNA Complexes by Capillary Electrophoresis.- 12 Polyacrylamide Gel Electrophoresis of DNA/Protein Complexes.- 13 Detection of DNA Curvature Using Transverse Pore Gradient Polyacrylamide Gel Electrophoresis.

Erscheint lt. Verlag 7.11.2012
Reihe/Serie Springer Lab Manuals
Zusatzinfo XIII, 328 p.
Verlagsort Berlin
Sprache englisch
Maße 193 x 242 mm
Gewicht 668 g
Themenwelt Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Biochemie
Technik Umwelttechnik / Biotechnologie
Schlagworte Analytical Biochemistry • Analytische Biochemie • biochemistry • Chemistry • DNA • DNA/Protein interaction • DNA sequencing • electrophoreses • Elektrophorese • forensic sciences • Forensische Medizin • interaction • Microbiology • pathogen • Polyacrylamid-Gelelektrophorese • Preparation • Protein • Protein-DNA Interaktionen • RNA
ISBN-10 3-642-47763-1 / 3642477631
ISBN-13 978-3-642-47763-8 / 9783642477638
Zustand Neuware
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