Genetic Library Construction and Screening

Advanced Techniques and Applications

Buch | Softcover

XIII, 241 Seiten

2012 | 2002
Springer Berlin (Verlag)
978-3-642-47733-1 (ISBN)

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Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning - Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.

Erscheint lt. Verlag	6.11.2012
Reihe/Serie	Springer Lab Manuals
Zusatzinfo	XIII, 241 p.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	393 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	2-Hybrid • cDNA • Cloning • DNA • Gene Cloning • Gene Library • Genome • Hybridization • Molecular Biology • PCR • Polymerase chain reaction • RNA
ISBN-10	3-642-47733-X / 364247733X
ISBN-13	978-3-642-47733-1 / 9783642477331
Zustand	Neuware