Resin Microscopy and On-Section Immunocytochemistry
Springer Berlin (Verlag)
978-3-540-67277-7 (ISBN)
I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Protocols for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Protocols for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I Examples of Typical Resin Embedding Regimes for Immunocytochemistry.- I.1 Solid Tissue, Pellets and Agar Blocks - Immersion Fixation.- I.2 Solid Tissue - Light Perfusion Fixation.- I.3 Solid Tissue - Very Light Perfusion Fixation.- I.4 Cryosubstitution.- I.5 Project Planner.- Appendix II List of Suppliers.- EM General.- EM Apparatus.- Electron Microscopes.- Flow Cytometry.- References.
From the reviews of the second edition:
"This comprehensive and valuable book is a must-have for the immunohistochemical laboratory. It extensively covers the field of onsection immunocytochemistry, including epoxy and acrylic resins, a variety of embedding and immunolabelling protocols ... . It is the only book I know covering LR-White and -Gold, the different Lowicryls, and Unicryl in this comprehensive manner. ... it is a very helpful book and because of its extensive theoretical and practical reference a standard for the beginner as well as those already familiar with immunocytochemistry." (Jens Krieger, Microscopy and Analysis, September, 2002)
Erscheint lt. Verlag | 10.4.2001 |
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Reihe/Serie | Springer Lab Manuals |
Zusatzinfo | XIX, 273 p. |
Verlagsort | Berlin |
Sprache | englisch |
Maße | 210 x 279 mm |
Gewicht | 710 g |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | crytotechniques • electron microscopy • immunolabelling • Immunologie • Ionenaustauscher • Mikroskopie • resin embedding • tissue • tissue fixation • Zytologie |
ISBN-10 | 3-540-67277-X / 354067277X |
ISBN-13 | 978-3-540-67277-7 / 9783540672777 |
Zustand | Neuware |
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