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Gigaseal Formation in Patch Clamping

With Applications of Nanotechnology

Majid Malboubi, Kyle Jiang (Autoren)

Buch | Softcover

XI, 94 Seiten

2013 | 2014
Springer Berlin (Verlag)
978-3-642-39127-9 (ISBN)

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This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.

Chapter 1 Introduction .-Chapter 2 Development of patch clamping .-
Chapter 3 Gigaseal formation .-
Chapter 4 Effect of roughness on gigaseal formation .-
Chapter 5 Effect of hydrophilicIty on gigaseal formation .-
Chapter 6 Effect of tip size on gigaseal formation .-
Chapter 7 Study of glass micropipettes from tip formation to characterization .-
References.

"This is a very good book on patch clamp protocol with a focus on the engineering and physics of pipettes. A description on tuning for bore diameter and various approaches to the culture dishes is detailed. ... This is a reliable resource for use in the physiology labs. Physiologists, students, postdocs, & senior investigators will find this very helpful." (Joseph J. Grenier, Amazon.com, September, 2015)

Erscheint lt. Verlag	7.8.2013
Reihe/Serie	SpringerBriefs in Applied Sciences and Technology
Zusatzinfo	XI, 94 p. 81 illus., 65 illus. in color.
Verlagsort	Berlin
Sprache	englisch
Maße	155 x 235 mm
Gewicht	177 g
Themenwelt	Medizin / Pharmazie ► Physiotherapie / Ergotherapie ► Orthopädie
Themenwelt	Technik ► Elektrotechnik / Energietechnik
Schlagworte	Gigaseal Formation using micropipettes • High Resistance Seals • high throughput screening • Hydrophilicity • nanotechnology • Patch Clamp • Patch clamping • Surface Roughness • Tip Size
ISBN-10	3-642-39127-3 / 3642391273
ISBN-13	978-3-642-39127-9 / 9783642391279
Zustand	Neuware