Human Embryonic Stem Cells (eBook)

The Practical Handbook

Stephen Sullivan, Chad Cowan, Kevin Eggan (Herausgeber)

eBook Download: PDF

2007 | 1. Auflage
424 Seiten
John Wiley & Sons (Verlag)
978-0-470-51159-6 (ISBN)

Infos

With this valuable practical guide, three members of the Harvard
Stem Cell Institute have compiled and edited the definite handbook
for the exciting new field of human embryonic stem cell research.
The editors have gathered protocols from scientists with extensive
reputation and expertise, describing and comparing currently used
techniques for the culture of human stem cells and discussing the
strengths and weaknesses of the different approaches.

Human Embryonic Stem Cells: The Practical
Handbook contains the first centralised collection of
methods used in human embryonic stem cell biology. The book covers
the derivation of human stem cell lines, the obtaining of cells
from human stem cell banks, the culturing and characterisation of
the cells, and the differentiation of the cells in vitro and
in vivo. Lastly, almost all of these protocols can also be
used for analyzing and manipulating induced pluripotency iPS stem
cells. This allows an even greater number of opportunities for
those interested in purusing work in pluripotent stem cells,
disease modelling, and other aspects of basic regenerative medicine
research.

The novel and useful focus of this book sets it apart from other
available books:

* Compares and evaluates the protocols used in leading
laboratories working on human embryonic stem cells

* Centred solely on practical protocols for human (not mouse)
embryonic stem cell research

* Includes extensive troubleshooting sections

* Addresses the different proclivities and behaviours of
individual human embryonic cell lines

* Contains techniques currently known only to a small number of
specialised laboratories worldwide

This handbook represents an essential source of up-to-date
practical information for all cell and developmental biologists
working with human embryonic stem cells or wishing to enter the
field. It is also essential reading for clinical researchers
in areas such as diabetes, cardiovascular disease, and neurological
diseases.

Praise from the reviews:

"...a highly readable and useful book... A notable
feature of the book is its air of openness and honesty... This
book... will help many to navigate the uncharted waters of
human embryonic stem cell biology." BRITISH SOCIETY FOR CELL
BIOLOGY

"... the imaginative solutions in this book can inspire us to
get past our most frustrating limitations." CELL STEM CELL

"... the richness in the details of each protocol presented
will certainly encourage more scientists to begin studies of Human
pluripotent stem cells..." REGENERATIVE MEDICINE

"In this fast-moving field, this [handbook] will help drive
advances of more and more researchers."
DIFFERENTIATION

"...a valuable resource for seasoned and novice
researchers... an excellent addition to the reference collection of
any medical library or research laboratory." THE AMERICAN
MEDICAL ASSOCIATION

Autor

Stephen Sullivan: Trinity College, Dublin, Ireland Kevin Eggan: Harvard University, Cambridge, MA, USA Chad A. Cowan: Harvard University, Cambridge, MA, USA

Inhalt

Foreword. Davor Solter

Preface.

Section 1: Obtaining and culturing human embryonic stem
cells.

1 Organization and good aseptic technique in the human embryonic
stem cell laboratory.

Minal J Patel, Emma L Stephenson, and Stephen L Minger

2 Sourcing human embryonic stem cell lines.

Glyn Stacey

3 Human embryo culture for human embryonic stem cell
derivation.

R. Douglas Powers and Jeannine Witmyer

4 Derivation of human embryonic stem cell lines.

Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton,
Kevin Eggan and Chad A. Cowan

5 Standard culture of human embryonic stem cells.

Jeremy M. Crook, Rachel Horne, and Alan Colman

6 Culture of human embryonic stem cells in chemically defined
conditions.

Julie Hsu Clark and Sheng Ding

Section 2: Characterization of undifferentiated embryonic stem
cells.

7 Phenotypic analysis of human embryonic stem cells.

Jonathan S. Draper, Cheryle A. Séguin and Peter W.
Andrews

8 Genetic and epigenetic analysis of human embryonic stem
cells.

Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova

Section 3: Manipulation of human embryonic stem cells.

9 In vivo differentiation of human embryonic stem
cells.

Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou

10 In vitro differentiation of human embryonic stem
cells.

A. Henry Sathananthan and Alan Trounson

11 Differentiation of human embryonic stem cells into
extraembryonic cell types.

Cheryle A. Séguin and Jonathan S. Draper

12 Directed differentiation of human embryonic stem cells
into:

(a) Early endoderm cells.

Kenji Osafune, Alice E. Chen and Douglas A. Melton

(b) Hepatic cells.

Neta Lavon and Nissim Benvenisty

(c) Pancreatic cells.

Hiram Chipperfield

13 Directed differentiation of human embryonic stem cells
into:

(a) Cardiomyocytes.

Christine Mummery, Robert Passier and Chris Denning

(b) Endothelial cells.

Carrie Soukup, Shulamit Levenberg and Ondine Cleaver

(c) Osteogenic cells.

Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali
Khademhosseini and Robert Langer

(d) Hematopoietic in vivo repopulating cells.

Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q.
Daley

(e) Lymphocytes.

Petter S. Woll and Dan S. Kaufman

(f) Myeloid cells.

Chantal Cerdan and Mickie Bhatia

14 Directed differentiation of human embryonic stem cells
into:

(a) Forebrain neurons.

Emily A. Davis and Lawrence S.B. Goldstein

(b) Dopaminergic neuron.

Jan Pruszak and Ole Isacson

(c) Spinal motor neurons.

Bao-Yang Hu and Su-Chun Zhang

15 (a) Gene targeting: knock out and knock in by
homologous recombination.

Thomas P. Zwaka

(b) RNA interference in human embryonic stem cells.

M. William Lensch, Asmin Tulpule and Holm Zaehres

(c) Generation of gene reporters using bacterial artificial
chromosome recombineering. Andrew J. Washkowitz and David A.
Shaywitz

Afterword. Azim Surani

Index.

Rezensionen

"A highly readable and useful book ... .[It] will help many to
navigate the uncharted waters of human embryonic stem cell
biology." (The British Society for Cell Biology
Newsletter, Autumn, 2008)

"[It] is a valuable resource for seasoned and novice
researchers; it would make an excellent addition to the reference
collection of any medical library or research laboratory."
(American Medical Association, September 2008)

"...the imaginative solutions in this book can inspire us to get
past our most frustrating limitations." (Cell Stem Cell,
September 2007)

"...the richness in the details of each protocol presented will
certainly encourage more scientists to begin studies of Human ES
cells..." (Regenerative Medicine, 2007)

"In this fast-moving field, this [handbook] will help drive
advances of more and more researchers."
(Differentiation, 2007)

"A highly readable and useful book ... [This book] will help
many to navigate the uncharted waters of human embryonic stem cell
biology." (British Society for Cell Biology, Autumn,
2008)

Produkt-Details

• Erscheint lt. Verlag: 2.8.2008
• Sprache: englisch
• Themenwelt: Medizin / Pharmazie
• Themenwelt: Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
• Themenwelt: Technik
• Schlagworte: Animal Development • Biotechnologie • Biotechnologie i. d. Biowissenschaften • Biotechnology • Biowissenschaften • Cell & Molecular Biology • Genetics • Genetik • Life Sciences • Stammzelle • Zellbiologie • Zell- u. Molekularbiologie • Zoologie / Individualentwicklung
• ISBN-10: 0-470-51159-1 / 0470511591
• ISBN-13: 978-0-470-51159-6 / 9780470511596