Drosophila Cells in Culture (eBook)

Guy Echalier (Autor)

eBook Download: PDF

1997 | 1. Auflage
702 Seiten
Elsevier Science (Verlag)
978-0-08-053053-6 (ISBN)

Infos

Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements.

Key Features
* Some thirty years of experience in handling in vitro cultured Drosophila cells
* Cell cultures which provide material for a multiplicity of biochemical approaches
* DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation
* Drosophila cell lines which qualify them for use in biotechnology

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Currently Drosophila is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of in vitro cultured Drosophila cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements. - Some thirty years of experience in handling in vitro cultured Drosophila cells- Cell cultures which provide material for a multiplicity of biochemical approaches- DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation- Drosophila cell lines which qualify them for use in biotechnology

Inhalt

Front Cover 1
Drosophila Cells in Culture 4
Copyright Page 5
Contents 8
Introduction 20
Chapter 1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells 34
I. Main Characteristics of the "Milieu Intérieur" of Drosophila 36
II. Description and Comparison of the Main Culture Media Available 45
III. Buffered Saline Solutions for Drosophila Tissues 60
IV. General Discussion 63
V. Prospects 88
Appendix 1 91
References 96
Chapter 2. Primary Cell Cultures of Drosophila Cells 102
I. Setting up Primary Cultures 104
II. Evolution of Primary Cultures from Disaggregated Wild-Type Embryos: Characteristics of Main Cell Types 112
III. Primary Cultures of Muscle Cells 121
IV. Nerve Cells in Primary Cultures 129
V. In Vitro Differentiation of Imaginal Cells: Hormonal Control 141
VI. Cell Cultures from Embryonic (Lethal) Mutants 146
Appendix 2 150
References 159
Chapter 3. Drosophila Continuous Cell Lines 162
I. Establishing Embryonic Cell Lines 164
II. Maintenance of Cell Lines 172
III. Catalog of Established Drosophila Embryonic Cell Lines 176
IV. Cell Lines from Larval or Imaginal Tissues 192
V. Cell Counting and Viability Determination 196
VI. Cell Cloning 198
VII. Storage by Freezing 201
VIII. Cell Fusion and Selection of Somatic Hybrids 204
IX. Living Cell Transportation 209
Appendix 3 210
References 218
Chapter 4. Karyotype and Cell Cycle 220
I. Karyotype Analysis 221
II. Cell Growth and Division Cycle 235
III. Haploid Cell Lines and Acentriolar Status of One of Them 246
Appendix 4 253
References 258
Chapter 5. Biology and Biochemistry of Cultured Cell Lines: 1. Nucleic Acids 260
I. Nucleic Acid Metabolism: Biosynthesis of Nucleoside Triphosphates 261
II. Ribosomal RNAs 269
III. Mitochondrial Genome 278
IV. DNA Repair and Sensitivity to Mutagens 284
V. Transcription Apparatus and Regulatory Factors 296
Appendix 5 307
References 309
Chapter 6. Biology and Biochemistry of Cultured Cell Lines: 2. Proteins 312
I. Cell Surface Glycoproteins: Adhesion/Recognition Molecules 313
II. Growth Factors, Receptors, and Protooncogenes 341
III. Drosophila Humoral Defenses 351
IV. Other Proteins or Enzymatic Activities 359
References 364
Chapter 7. Experimental Models of Gene Regulation: 1. Heat-Shock Response of Drosophila Cells 368
I. The Heat-Shock System 370
II. Major Heat-Shock Proteins and Their Encoding Genes 371
III. Heat-Shock Protein Induction at Transcriptional Level 378
IV. Translational Control in Heat Shock 382
V. Recovery from Heat Shock 385
VI. Other Temperature-Inducible Genes 387
VII. Cell Responses to Different Stresses 393
VIII. Intracellular Localizations and Putative Functions of Heat-Shock Proteins 396
IX. Regulatory Regions of hsp Genes and Transcriptional Activators 405
References 422
Chapter 8. Experimental Models of Gene Regulation: 2. Cell Responses to Hormone 426
I. Hormonal Stimulus 430
II. Repertoire of Cell Responses 435
III. "Maturation" Phase: A Critical Period for Hormone Action 459
IV. Cell Resistance to Ecdysteroids 462
V. Ecdysteroid Receptors 463
VI. Functional Interference with Juvenile Hormone 466
VII. Validity of Model and Its Current Interest 468
References 470
Chapter 9. Gene Transfer into Cultured Drosophila Cells 472
I. Cell Transformation 475
II. Transient Expression 481
III. Recipient Cell Lines 488
IV. Transfection Methods 493
V. Selection Systems 499
VI. Efficient Promoters 508
VII. Reporter Genes 519
VIII. Shuttle Vectors 523
IX. Prospects 524
Appendix 9 529
References 541
Chapter 10. Transposons 546
I. Long Terminal Repeat Retrotransposons: copia-like Elements 547
II. Line-Like Retroposons 575
III. Bacterial Insertion-like Transposons: P Element 581
References 585
Chapter 11. Drosophila Viruses and Other Infections of Cultured Cells 588
I. Drosophila Hereditary Sigma Virus and Persistent Infection with Various Arboviruses 589
II. Endogenous Viruses of Drosophila and Cell Lines 601
III. Looking for Efficient Viral Promoters in Drosophila Cell Lines 609
IV. Experimental Studies of Parasite–Host Cell Relations in Drosophila Cell Lines 617
Appendix 11 623
References 627
Bibliography 630
Index 724

Produkt-Details

• Erscheint lt. Verlag: 24.2.1997
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Anatomie / Neuroanatomie
• Themenwelt: Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zellbiologie
• Themenwelt: Naturwissenschaften ► Biologie ► Zoologie
• Themenwelt: Technik ► Umwelttechnik / Biotechnologie
• ISBN-10: 0-08-053053-2 / 0080530532
• ISBN-13: 978-0-08-053053-6 / 9780080530536