Molecular and Cellular Methods in Developmental Toxicology

George P. Daston (Autor)

Buch | Softcover

304 Seiten

1996
Crc Press Inc (Verlag)
978-0-8493-3342-2 (ISBN)

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This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

Daston, George P.

Part I. Molecular Techniques -- Chapter 1. Molecular Techniques for Developmental Toxicology: An Introduction/George P Daston -- Chapter 2. Visualization of Gene Expression Patterns by In Situ Hybridization/ Urs Albrecht, Gregor Eichele, Jill A. Helms, and Hui-Chen Lu -- Chapter 3. Utilizing Transgenic Mice to Study Gene Regulation and Function/Michael R. Blackburn and Rodney E. Kellems -- Chapter 4. The Use of Antisense Oligonucleotide Technology and Mouse Whole-Embryo Culture to Study Gene Function During Organogenesis/Linda Foerst Potts and T W. Sadler -- Chapter 5. Nucleic Acid Amplification Technologies/Richard H. Finnell, Scott J. Vacha, and Scott A. Mackler -- Part II. Protein Identification -- Chapter 6. Fundamentals of Protein Identification: Biochemical and Immunological Analysis of Developmentally Altered Protein Expression/Gerald B. Grunwald -- Chapter 7. Immunohistochemistry/Philip E. Mirkes and Sally A Little -- Part III. Cellular Techniques -- Chapter 8. Cellular Techniques for Teratological Cell Death/Thomas B. Knudsen -- Chapter 9. Applications of Flow Cytometry in Developmental and Reproductive Toxicology/Kenneth H. Elstein and Robert M. Zucker -- Chapter 10. Modern Techniques for Cell Labeling in Avian and Murine Embryos/Diana K. Darnell and Gary C. Schoenwolf -- Index.

Erscheint lt. Verlag	26.9.1996
Reihe/Serie	Methods in Life Sciences - Toxicology Section
Zusatzinfo	9 Tables, black and white
Verlagsort	Bosa Roca
Sprache	englisch
Maße	156 x 234 mm
Gewicht	521 g
Einbandart	Paperback
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Gynäkologie / Geburtshilfe
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	0-8493-3342-3 / 0849333423
ISBN-13	978-0-8493-3342-2 / 9780849333422
Zustand	Neuware