Hematopoietic Stem Cell Protocols
Humana Press Inc. (Verlag)
978-1-61737-816-4 (ISBN)
Overview.- The Expanding Tool Kit for Hematopoietic Stem Cell Research.- Stem Cell Enrichment and Analysis.- Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties.- Mobilization of Hematopoietic Stem and Progenitor Cells in Mice.- Transmigration of Human CD34+ Cells.- Flow Cytometry-Based Cell Cycle Measurement of Mouse Hematopoietic Stem and Progenitor Cells.- Analysis of Apoptosis in Hematopoietic Stem Cells by Flow Cytometry.- In Vitro Assays and Differentiation.- In Vitro Hematopoietic Differentiation of Murine Embryonic Stem Cells.- Hematopoietic Development of Human Embryonic Stem Cells in Culture.- In Vitro Human T Cell Development Directed by Notch–Ligand Interactions.- In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C.- Transplantation Assays for Mouse and Human HSC.- Hematopoietic Stem Cell Transplant in Mice by Intra-Femoral Injection.- Hematopoietic Stem Cell Transplant into Non-Myeloablated W/W v Mice to Detect Steady-State Engraftment Defects.- Isolation and Functional Characterization of Side Population Stem Cells.- Transplantation of Chimeric Fetal Liver to Study Hematopoiesis.- Immunodeficient Mouse Models to Study Human Stem Cell-Mediated Tissue Repair.- Genetic Modification of HSCs and Imaging Engraftment.- Retroviral Transduction of Murine Hematopoietic Stem Cells.- Lentiviral Gene Transduction of Mouse and Human Stem Cells.- Retroviral Integration Site Analysis in Hematopoietic Stem Cells.- A Detailed Protocol for Bacterial Artificial Chromosome Recombineering to Study Essential Genes in Stem Cells.- Bioluminescence Imaging of Hematopoietic Stem Cell Repopulation in Murine Models.
Erscheint lt. Verlag | 16.12.2010 |
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Reihe/Serie | Methods in Molecular Biology ; 430 |
Zusatzinfo | XIV, 312 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 155 x 235 mm |
Themenwelt | Medizinische Fachgebiete ► Innere Medizin ► Hämatologie |
Studium ► 1. Studienabschnitt (Vorklinik) ► Physiologie | |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Naturwissenschaften ► Biologie ► Zellbiologie | |
ISBN-10 | 1-61737-816-X / 161737816X |
ISBN-13 | 978-1-61737-816-4 / 9781617378164 |
Zustand | Neuware |
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