Human Cell Culture Protocols

Buch | Softcover

360 Seiten

2010 | Softcover reprint of hardcover 2nd ed. 2005
Humana Press Inc. (Verlag)
978-1-61737-431-9 (ISBN)

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A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Establishment and Maintenance of Normal Human Keratinocyte Cultures.- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters.- Isolation and Culture of Human Osteoblasts.- Human Osteoclast Culture From Peripheral Blood Monocytes.- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation.- Human Myoblasts and Muscle-Derived SP Cells.- Cell Cultures of Autopsy-Derived Fibroblasts.- Primary Culture and Differentiation of Human Adipocyte Precursor Cells.- Human Mononuclear Phagocytes in Tissue Culture.- Purification of Peripheral Blood Natural Killer Cells.- Human Fetal Brain Cell Culture.- Culturing Human Schwann Cells.- Well-Differentiated Human Airway Epithelial Cell Cultures.- Isolation and Culture of Human Alveolar Epithelial Cells.- A New Approach to Primary Culture of Human Gastric Epithelium.- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach.- Isolation and Culture of Human Hepatocytes.- Glomerular Epithelial and Mesangial Cell Culture and Characterization.- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules.- Culture of Parathyroid Cells.- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media.- Primary Culture of Human Antral Endocrine and Epithelial Cells.- Conjunctiva Organ and Cell Culture.- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Medicine ; 107
Zusatzinfo	120 Illustrations, black and white; XII, 360 p. 120 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Studium ► 1. Studienabschnitt (Vorklinik) ► Histologie / Embryologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-431-8 / 1617374318
ISBN-13	978-1-61737-431-9 / 9781617374319
Zustand	Neuware