Apoptosis Methods in Pharmacology and Toxicology

Approaches to Measurement and Quantification

Buch | Softcover

156 Seiten

2011
Humana Press Inc. (Verlag)
978-1-61737-239-1 (ISBN)

Artikel merken

Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.

Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death.- Analysis of Apoptosis by Laser-Scanning Cytometry.- Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections.- DNA Microarrays.- Microarray Analysis of Apoptosis.- ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases.

"The book emphasizes techniques that can be used to quantify apoptosis, such as cell morphology, flow cytometry, laser-scanning cytometry, ELISA, and DNA microarray. The flow cytometry and ELISA sections are the best, as they are applicable to a wide range of laboratories because of their ease of use do not require any extraordinary facilities. . .The book is well written and instructive, and the techniques described can be applied easily to topics in pharmacology and toxicology as well as in general apoptosis research. A strong point is that the authors emphasize the need to combine morphological assessment of the cell with other apoptotic parameters in order to fully characterize a cell as apoptotic."-Doody's Health Science Book Review Journal

Reihe/Serie	Methods in Pharmacology and Toxicology
Zusatzinfo	XIV, 156 p.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie
Themenwelt	Medizin / Pharmazie ► Pharmazie
ISBN-10	1-61737-239-0 / 1617372390
ISBN-13	978-1-61737-239-1 / 9781617372391
Zustand	Neuware