DNA Topoisomerase Protocols
Humana Press Inc. (Verlag)
978-1-61737-057-1 (ISBN)
Assaying DNA Topoisomerase I Relaxation Activity.- DNA Topoisomerase II-Catalyzed DNA Decatenation.- Plasmid DNA Supercoiling by DNA Gyrase.- Analyzing Reverse Gyrase Activity.- Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography.- Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II.- Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay.- Filter Binding Assays for Topoisomerase—DNA Complexes.- DNATopoisomerase I-Mediated Nicking of Circular Duplex DNA.- Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives.- Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation.- Synthesis and Use of DNA Containing a 5?-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases.- Isolation of Covalent Enzyme-DNA Complexes.- ICE Bioassay.- DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I.- Drug—DNA Interactions.- Quinolone Interactions with DNA and DNA Gyrase.- Bactericidal Assays for Fluoroquinolones.- Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I.- Drug-Induced Cytotoxicity in Tissue Culture.- Analysis of Drug Uptake-Flow Cytometry.- Cell-Cycle Analysis of Drug-Treated Cells.- Assaying Drug-Induced Apoptosis.- Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II.- PCR-Based Cloning of DNA Topoisomerase Genes.- Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA.- Topoisomerase II-Mediated Cleavage of Plasmid DNA.- Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates.- Studying DNATopoisomerase I-Targeted Drugs In the Yeast.- Yeast Systems for Demonstrating the Targets of Anti-Topoisomerase II Agents.
Erscheint lt. Verlag | 10.11.2010 |
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Reihe/Serie | Methods in Molecular Biology ; 95 |
Zusatzinfo | XIV, 334 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 152 x 229 mm |
Themenwelt | Medizin / Pharmazie ► Pflege |
Medizin / Pharmazie ► Pharmazie ► PTA / PKA | |
ISBN-10 | 1-61737-057-6 / 1617370576 |
ISBN-13 | 978-1-61737-057-1 / 9781617370571 |
Zustand | Neuware |
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