Peptide Arrays on Membrane Supports
Springer Berlin (Verlag)
978-3-642-07639-8 (ISBN)
The technique of oligopeptide synthesis on a cellulose membrane and the subsequent binding assays allow the investigation of protein interactions. A particular advantage of these peptide arrays (SPOT - technology) is the high number of oligopeptide probes that can be tested in parallel. Detailed protocols for peptide synthesis, and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding.
Dr. Joachim Koch hat bei Prof. Dr. Markus Rudolf am Dresdner Bank Stiftungslehrstuhl für Finanzwirtschaft WHU - Otto Beisheim Hochschule in Vallendar promoviert. Derzeit arbeitet er in der Investment Banking Division von der UBS AG in Frankfurt
1 SPOT Synthesis - Scope of Applications.- 2 Chemistry of Fmoc Peptide Synthesis on Membranes.- 3 Manual Peptide Synthesis.- 4 Automated Synthesis of Solid-Phase Bound Peptides.- 5 Epitope Mapping of Antibodies with Solid-Phase Oligopeptides.- 6 Protein-Protein Interactions.- 7 Analysis of Protein-DNA Interactions.- 8 Affinity Purification and Competition Assays Using Solid-Phase Oligopeptides.- 9 Mutational Analysis and Structure Predictions.- 10 Modification of Immobilized Peptides.- 11 Immobilized Peptides to Study Protein-Protein Interactions - Potential and Pitfalls.- Abbreviations.
From the reviews:
"In this book the authors provide a detailed overview of the technology, including insights into basic chemistry and established applications. All protocols have been written by experienced researchers in SPOT technology and have therefore been optimized for practical use. ... It is ideally suited not only for basic research laboratories but also for diagnostic and therapeutic applications since many diseases are related to dysfunctions in protein recognition and binding." (New Drugs, Issue 7, October, 2002)
"Detailed protocols for peptide synthesis and the analysis of protein-protein, protein-DNA interactions as well as epitope mapping are presented in this manual. It is ideally suited not only for basic research laboratories but potentially also for diagnostic and therapeutic applications ... . This book provides a detailed overview of the technology, including insights into the basic chemistry and established applications. ... The book is suitable for researchers in basic research and in therapeutic applications as well as for advanced students." (Biotech International, Vol. 15 (2), 2003)
| Erscheint lt. Verlag | 21.10.2010 |
|---|---|
| Reihe/Serie | Springer Lab Manuals |
| Zusatzinfo | X, 173 p. 23 illus. |
| Verlagsort | Berlin |
| Sprache | englisch |
| Maße | 155 x 235 mm |
| Gewicht | 285 g |
| Themenwelt | Medizin / Pharmazie ► Medizinische Fachgebiete ► Pharmakologie / Pharmakotherapie |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Schlagworte | binding site • DNA • epitope-mapping • Epitopkartierung • Peptide synthesis • Peptidsynthese • Proteine • protein interaction • Proteininteraktionen • proteins • signal transduction |
| ISBN-10 | 3-642-07639-4 / 3642076394 |
| ISBN-13 | 978-3-642-07639-8 / 9783642076398 |
| Zustand | Neuware |
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