Laboratory Methods for the Detection of Mutations and Polymorphisms in DNA

Graham R. Taylor (Autor)

Buch | Hardcover

352 Seiten

1997
Crc Press Inc (Verlag)
978-0-8493-9233-7 (ISBN)

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Deals with the experimental analysis of DNA and presents various protocols. This book details various techniques from the simple to the highly complex. It is suitable for researchers working in human, animal, or plant molecular genetics and for hospital and commercial laboratories.

The analysis of DNA sequence polymorphisms and mutations is of central importance in understanding biological systems. This book is devoted to the experimental analysis of DNA and presents easy-to-follow protocols. Various techniques from the simple to the highly complex are detailed in this volume, providing a wide spectrum of available methods and practical advice. The methods are described in terms of:
History and background

Principles and theory

Equipment and reagents

Protocols

Troubleshooting

Applications

Improvements

Results

Comparisons with other methods

Future prospects and developments
This is an essential manual for researchers working in human, animal, or plant molecular genetics and is particularly valuable for hospital and commercial laboratories.

Taylor/, Graham R.

Introduction, G.R. Taylor
Arbitrarily Primed PCR Fingerprints, F. Mathieu-Daude, D. Ralph, and M. McClelland
Minisatellite Analysis, G. Rysiecki, W.P. Childs, and N. Duxbury
Microsatellite Analysis, S. Payne
ARMS Analysis of Point Mutations, S. Little
Solid Phase Minisequencing, A.C. Syvanen
Oligonucleotide Ligation Assay, E.S. Winn-Deen
Combined Single-Strand Conformation Polymorphism and Heteroduplex Analysis, A.J. Wallace
Denaturing Gradient Gel Electrophoresis (DGGE), M. Loosekoot and R. Fodde
Coupled Transcription-Translation, T. Hamzehleoi and S. West
Peptide Nucleic Acids, H. Orum, T Koch, M. Egholm, H. O'Keefe, and J. Coull
Orphan Peak Analysis, M. Hattori, H. Hagiwara, and Y. Sakaki
Sequence-Specific Gene Signatures Produced by LSSP-PCR, A.J.G. Simpson and S.D.J. Pena
Bisulphite Genomic Sequencing of Methylated Cytosines, S.J. Clark and M. Frommer
Direct Sequencing of PCR Products, E. Werle
Heterozygote Determination Using Automated DNA Sequencing Technology, M.N. Kronick
A Robust Sequencing Protocol for PCR Products, M.D. Robinson and K.J. Leach
Use of MutY and Thymine Glycosylase to Detect Point Mutations, I.C. Hsu
MutS-Exonuclease Protection for the Detection of Mismatched Heteroduplexes, V.H. Laitinen, G.R. Taylor, S. Hamilton and P. Flick
Enzyme Mismatch Cleavage-Bacterial Resolvases, R.D. Mashal and J. Sklar
Chemical Cleavage of Mismatch in Heteroduplexes, J. Prosser
Comparison of Mutation Detection by Chemical Cleavage of Mismatch (CCM) and Enzyme Mismatch Cleavage (EMC), R. Youil, S.J. Ramus, and R.G.H. Cotton
Analysis of DNA Sequence Using Biosensors, P. Nilsson, B. Persson, M. Uhlen, and P.A. Nygren
Repeat Expansion Detection, C. Zander, C. Erickson Burgess, J. Johansen, K. Lindblad, T. Hudson, and M. Schalling
Comparative Genomic Hybridization, A Kallioniemi, R. Karhu, and O.-P. Kallioniemi
Large-Scale Electrophoretic Studies: Microplate Array Diagonal Gel Electrophoresis (MADGE) and Profiling of Oligonucleotide Dissociation by Gel Electrophoresis (PODGE), I.N.M. Day, S.D. O'Dell, G.P. Weavind, and S.E. Humphries
DNA Sequence Database Searches, Comparisons, and Primer Design, G. Williams
Accessing Genome Data: The Human Genome Database, Internet, and WWW, G. Williams
List of Suppliers
Index

Erscheint lt. Verlag	24.1.1997
Zusatzinfo	1 Halftones, black and white; 21 Tables, black and white
Verlagsort	Bosa Roca
Sprache	englisch
Maße	178 x 254 mm
Gewicht	982 g
Einbandart	gebunden
Themenwelt	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
ISBN-10	0-8493-9233-0 / 0849392330
ISBN-13	978-0-8493-9233-7 / 9780849392337
Zustand	Neuware