Nanopore Sequencing

Methods and Protocols

Buch | Softcover

324 Seiten

2024 | 1st ed. 2023
Springer-Verlag New York Inc.
978-1-0716-2998-7 (ISBN)

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This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.

The current state of nanopore sequencing.- Hybrid genome assembly of short and long reads in Galaxy.- Microbial genome sequencing and assembly using nanopore sequencers.- De novo genome assembly of Japanese Black cattle as model of an economically relevant animal.- How to sequence and assemble plant genomes.- Detection of DNA modification using nanopore sequencers.- Ultra-low input genome library preparation for nanopore sequencing with droplet-MDA.- The method of eliminating the Wolbachia endosymbiont genomes from insect samples prior to long-reads.- A nanopore sequencing course for graduate school curriculum.- A guide to genome sequencing containing ultra-long repetitive regions.- Analysis of tandem repeat expansions using long DNA reads.- Finding rearrangements in nanopore DNA reads with last and dnarrange..- Long read whole-genome sequencing using a nanopore sequencer and detection of structural variants in cancer genomes.- Full-length 16S rRNA gene analysis using long-read nanopore sequencing for rapid identification of bacteria from clinical specimens.- Nanopore sequencing data analysis of 16S rRNA genes using Genome Sync-GSTK system.- Genomic epidemiological analysis of antimicrobial-resistant bacteria with nanopore sequencing.- Rapid and comprehensive identification of nontuberculous mycobacteria.- Long-read single-cell sequencing using scCOLOR-seq.- Unfolding the Bacterial Transcriptome Landscape using Oxford Nanopore Technology Direct RNA Sequencing.- Nanopore Direct RNA Sequencing of Monosome-and Polysome-bound RNA.- RNA modification detection using nanopore direct RNA sequencing and nanoDoc2.

Erscheinungsdatum	16.02.2024
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	63 Illustrations, color; 19 Illustrations, black and white; XI, 324 p. 82 illus., 63 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Mathematik / Informatik ► Informatik ► Theorie / Studium
	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Technik ► Umwelttechnik / Biotechnologie
Schlagworte	Bacterial genomes • Genome sequencing • long repetitive genes • Nanopore sequencers • structural variants
ISBN-10	1-0716-2998-0 / 1071629980
ISBN-13	978-1-0716-2998-7 / 9781071629987
Zustand	Neuware