Plant Metabolic Engineering

Methods and Protocols

Buch | Hardcover

244 Seiten

2021 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-1821-9 (ISBN)

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This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering.

Methods for the Development of Recombinant Microorganisms for the Production of Natural Products.- Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus Byproducts.- Reconstitution of Metabolic Pathway in Nicotiana benthamiana.- A Protocol for Phylogenetic Reconstruction.- RNA-Seq Data Analysis Pipeline for Plants: Transcriptome Assembly, Alignment, and Differential Expression Analysis.- A Protocol for Horizontally Acquired Metabolic Gene Detection in Algae.- Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction Cartridges.- Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization High Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L.) Berry.- GC-MS/MS Profiling of Plant Metabolites.- Analysis of Grape Volatiles using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based Metabolomics.- A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites.- Macrolipidomic Profiling of Vegetable Oils: The Analysis of Sunflower Oils with Different Oleic Acid Content.- Non-Targeted Lipidomics using a Robust and Reproducible Lipid Separation using UPLC with Charged Surface Hybrid Technology and High Resolution Mass Spectrometry.- Comprehensive Analysis of Plant Lipids using Sub-2µm Particle CO2-Based Chromatography Coupled to Mass Spectrometry.- Bioinformatics in Lipidomics: Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid Software.- A Protocol for Prion Discovery in Plants.- Structural Determination of Uridine Diphosphate Glycosyltransferases using X-Ray Crystallography.

Erscheinungsdatum	22.11.2021
Reihe/Serie	Methods in Molecular Biology ; 2396
Zusatzinfo	55 Illustrations, color; 14 Illustrations, black and white; X, 244 p. 69 illus., 55 illus. in color. With online files/update.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Mathematik / Informatik ► Informatik ► Theorie / Studium
	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	CRISPR • E.coli • Nicothiana benthamiana • Talen • X-ray crystallography
ISBN-10	1-0716-1821-0 / 1071618210
ISBN-13	978-1-0716-1821-9 / 9781071618219
Zustand	Neuware