Plant Genome Editing with CRISPR Systems

Methods and Protocols

Yiping Qi (Herausgeber)

Buch | Hardcover

367 Seiten

2019 | 1st ed. 2019
Humana Press Inc. (Verlag)
978-1-4939-8990-4 (ISBN)

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This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Plant DNA Repair Pathways and their Applications in Genome Editing.- Rapid Screening of CRISPR/Cas9-Induced Mutants using the ACT-PCR Method.- Decoding Sanger-Sequencing Chromatograms from CRISPR-Induced Mutations.- Creating Large Chromosomal Deletions in Rice using CRISPR/Cas9.- A Multiplexed CRISPR-Cas9 Editing System Based on the Endogenous tRNA Processing.- A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants.- CRISPR-Act2.0: An Improved Multiplexed System for Plant Transcriptional Activation.- Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9.- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9.- An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize.- Fluorescence Marker-Assisted Isolation of Cas9-Free and CRISPR-Edited Arabidopsis Plants.- Creating Targeted Gene Knock-Outs in Brassica oleracea using CRISPR/Cas9.- Application of CRISPR/Cas9-Mediated Gene Editing in Tomato.- Genome Editing in Potato with CRISPR/Cas9.- Visual Assay for Gene Editing using a CRISPR/Cas9 System in Carrot Cells.- Genome Editing in Soybean with CRISPR/Cas9.- Genome Editing in Citrus Tree with CRISPR/Cas9.- Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems.- Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1.- Targeted Mutagenesis using FnCpf1 in Tobacco.- Gene Replacement by Intron Targeting with CRISPR-Cas9.- Targeted Base Editing with CRISPR-Deaminase in Tomato.- Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System.- Biolistic Delivery of CRISPR/Cas9 with Ribonucleoprotein Complex in Wheat.- DNA-Free Genome Editing via Ribonucleoprotein (RNP) Delivery of CRISPR/Cas in Lettuce.- An Automated Protoplast Transformation System.

Erscheinungsdatum	25.01.2019
Reihe/Serie	Methods in Molecular Biology ; 1917
Zusatzinfo	65 Illustrations, color; 8 Illustrations, black and white; XV, 367 p. 73 illus., 65 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Botanik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	Arabidopsis • Cas9 genome • CRISPR • DeCoding Sanger sequencing data • Gene replacement
ISBN-10	1-4939-8990-1 / 1493989901
ISBN-13	978-1-4939-8990-4 / 9781493989904
Zustand	Neuware