RNA Scaffolds

Methods and Protocols

Buch | Softcover

231 Seiten

2016 | Softcover reprint of the original 1st ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-5589-3 (ISBN)

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This volume provides techniques on recent developments that use RNA scaffolds as molecular tools. RNA Scaffolds: Methods and Protocols guides readers through methods on various domains as molecular biology, nanotechnologies, and structural biology. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and authoritative, RNA Scaffolds: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

A Method to Predict the 3D Structure of an RNA Scaffold.- Post-crystallization Improvement of RNA Crystal Diffraction Quality.- Expression and Purification of RNA-protein Complexes in Escherichia coli.- Production of Homogeneous Recombinant RNA using a tRNA Scaffold and Hammerhead Ribozymes.- In vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold.- Detection of RNA-Protein Interactions using Tethered RNA Affinity Capture.- A Universal Method for Labeling Native RNA in Live Bacterial Cells.- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.- RNA Scaffold: Designed To Co-Localize Enzymes.- Artificial Ligase Ribozymes Isolated by a “Design And Selection” Strategy.- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach.- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells.- Design and Characterisation of Topological Small RNAs.- Folding RNA-Protein Complex into Designed Nanostructures.- Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60˚ to 90˚ or 108˚.- RNA-Mediated CdS Based Nanostructures.- An Effective Method for Specific Gene Silencing in Escherichia coli using Artificial Small RNA.

Erscheinungsdatum	31.10.2016
Reihe/Serie	Methods in Molecular Biology ; 1316
Zusatzinfo	41 Illustrations, color; 20 Illustrations, black and white; XIII, 231 p. 61 illus., 41 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Informatik ► Weitere Themen ► Bioinformatik
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	aptazymes • Data acquisition • DSL ribozyme • Nano-objects • potentialization of the silencing activity • riboswitches • RNA origami • RNA protein co-production in the bacteria • RNA structure data bank • Vfold
ISBN-10	1-4939-5589-6 / 1493955896
ISBN-13	978-1-4939-5589-3 / 9781493955893
Zustand	Neuware