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PCR Mutation Detection Protocols -

PCR Mutation Detection Protocols

Buch | Softcover
224 Seiten
2002
Humana Press Inc. (Verlag)
978-0-89603-617-8 (ISBN)
CHF 109,25 inkl. MwSt
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Provides biological and clinical investigators with a collection of PCR-based screening methods suitable for detecting the presence of both known and novel mutations. This book includes the techniques of DNA array analysis, along with experimental methods as conformation-sensitive gel electrophoresis.
Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Agarose and Polyacrylamide Gel Electrophoresis Andrea M. Guilliatt Internal Labeling of DNA Probes Ralph Rapley and Bimal D. M. Theophilus End-Labeling of DNA Probes Adrian J. Harwood Southern Blotting of Agarose Gels by Capillary Transfer Ralph Rapley and Ian J. Williams Restriction Fragment Length Polymorphism Mohammad S. Enayat PCR: Principles, Procedures, and Parameters Nicola Louise Jones Allele-Specific Oligonucleotide PCR Elaine K. Green Long-Range PCR, Peter A. Davies and George Gray Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing Anu Wartiovaara and Ann-Christine Syvanen Cycle Sequencing of PCR Products G. K. Surdhar Fluorescent In Situ Hybridization Sara A. Dyer and Elaine K. Green. The Protein Truncation Test Carol A. Hardy Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli Denaturing Gradient Gel Electrophoresis Yvonne Wallis Conformation-Sensitive Gel Electrophoresis Ian J. Williams and Anne C. Goodeve SSCP/Heteroduplex Analysis Andrew J. Wallace Cleavase(R) Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection Laura Heisler and Chao-Hung Lee Automated Genotyping Using the DNA MassArraya Technology Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Koster An Introduction to Bioinformatics Henry Brzeski

Erscheint lt. Verlag 4.4.2002
Reihe/Serie Methods in Molecular Biology ; v. 187
Zusatzinfo 1
Verlagsort Totowa, NJ
Sprache englisch
Maße 234 x 156 mm
Gewicht 321 g
Themenwelt Informatik Weitere Themen Bioinformatik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Mikrobiologie / Immunologie
ISBN-10 0-89603-617-0 / 0896036170
ISBN-13 978-0-89603-617-8 / 9780896036178
Zustand Neuware
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