Pathology, Vector Studies, and Culture (eBook)

Julius P. Kreier (Herausgeber)

eBook Download: PDF

2014 | 1. Auflage
346 Seiten
Elsevier Science (Verlag)
978-1-4832-5871-3 (ISBN)

Infos

Malaria: Volume 2, Pathology, Vector Studies, and Culture is a collection of papers that deals with erythrocyte destruction mechanism in malaria, the pathology of malaria, colonization of laboratory mosquitoes, and their transmission of plasmodia. Other papers describe the culture of the invertebrate stages of plasmodia, of mosquito tissues, and also of erythrocytic and exoerythrocytic stages of plasmodia. One paper constructs a model to show the roles of the different destructive and regenerative processes in the mechanisms of erythrocyte destruction in malaria. Another paper describes the organ changes and physiopathological mechanism connected with Plasmodium infection. These organs concern the spleen, liver, heart, and vascular system. One paper reviews the procedures involved in malaria transmission by mosquitoes, as well as some specialized procedures unique in avian, rodent, simian, and human malarias. Another paper discusses the benefits and drawbacks of a culture medium based on the composition of adult anopheline hemolymph. This medium should lead to a rapid growth rate in primary cultures of mosquito cells and a shorter interval of adaptation for continuous cell lines. The collection can prove useful for pharmacologists, general medical practitioners, investigators, and laboratory technicians involved in mosquito borne diseases or tropical medicine research.

Inhalt

Front Cover 1
Pathology, Vector Studies, and Culture 4
Copyright Page 5
Table of Contents 6
List of Contributors 10
Preface 12
Contents of Volumes 1 and 3 14
Contents of Babesiosis 16
Chapter 1. Erythrocyte Destruction Mechanisms in Malaria 20
I. INTRODUCTION 20
II. MECHANISMS OF ERYTHROCYTE DESTRUCTION 21
III. PROPOSED MODEL OF THE RELATIONSHIP BETWEEN ERYTHROCYTE LOSS AND PARASITEMIA 55
REFERENCES 57
Chapter 2. Pathology of Malaria 66
I. INTRODUCTION 66
II. SPLEEN PATHOLOGY 67
III. LIVER PATHOLOGY 73
IV. HEART AND VASCULAR PATHOLOGY 79
V. HEMATOPATHOLOGY 85
VI. PULMONARY PATHOLOGY 89
VII. THYMUS PATHOLOGY 94
VIII. RENAL PATHOLOGY 96
IX. NEUROPATHOLOGY 108
X. PLACENTA PATHOLOGY 112
REFERENCES 114
Chapter 3. Colonization and Maintenance of Mosquitoes in the Laboratory 122
I. INTRODUCTION 122
II. BASIC MOSQUITO BIOLOGY 124
III. COLLECTION OF FIELD MATERIAL 130
IV. TRANSPORT OF LIVING MATERIAL 136
V. REARING 141
VI. SELECTION PROCEDURES 158
VII. PATHOGEN AND PESTICIDE PROBLEMS 160
REFERENCES 163
Chapter 4. The Transmission by Mosquitoes of Plasmodia in the Laboratory 172
I. INTRODUCTION 173
II. SUSCEPTIBILITY OF MOSQUITOES TO PLASMODIUM 174
III. GAMETOCYTES IN THE VERTEBRATE HOST 177
IV. THE INFECTIVE BLOOD MEAL 191
V. MAINTENANCE OF INFECTED MOSQUITOES 193
VI. SAMPLING PROCEDURES FOR DETERMINING THE DEGREEOF MOSQUITO INFECTION 195
VII. HARVESTING, PURIFYING, AND MAINTAINING SPOROZOITES 202
VIII. INFECTING VERTEBRATES WITH SPOROZOITES 207
IX. QUANTITATION OF SPOROZOITE INFECTIVITY AND VIABILITY 208
X. SPECIFIC HOST-PARASITE-VECTOR SYSTEMS IN MALARIARESEARCH 212
XI. CONCLUSIONS 237
REFERENCES 237
Chapter 5. Culture of the Invertebrate Stages of Plasmodia and the Culture of Mosquito Tissues 254
I. INTRODUCTION 254
II. ATTEMPTS TO CULTURE THE INVERTEBRATE STAGES OF PLASMODIA 256
III. CURRENT METHODS OF INITIATING PRIMARY CELL AND ORGAN CULTURES FROM MOSQUITO SPECIES WITH EMPHASIS ON ANOPHELINES 270
IV. ADULT ANOPHELINE HEMOLYMPH ANALYSIS AND THE DESIGN OF CULTURE MEDIA 275
V. CONCLUDING REMARKS 282
REFERENCES 282
Chapter 6. Cultivation of Erythrocytic and Exoerythrocytic Stages of Plasmodia 290
I. INTRODUCTION 290
II. CULTURE OF ERYTHROCYTIC STAGES WITHIN THEIR HOST CELLS 292
III. EXTRACELLULAR CULTURE OF ERYTHROCYTIC STAGES 317
IV. EXOERYTHROCYTIC STAGES IN CULTURE 325
REFERENCES 332
Index 340

Produkt-Details

• Erscheint lt. Verlag: 12.5.2014
• Sprache: englisch
• Themenwelt: Sachbuch/Ratgeber ► Gesundheit / Leben / Psychologie ► Krankheiten / Heilverfahren
• Themenwelt: Mathematik / Informatik ► Mathematik
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Allgemeinmedizin
• Themenwelt: Medizin / Pharmazie ► Medizinische Fachgebiete ► Innere Medizin
• Themenwelt: Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
• ISBN-10: 1-4832-5871-8 / 1483258718
• ISBN-13: 978-1-4832-5871-3 / 9781483258713