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PCR Primer Design

Chhandak Basu (Herausgeber)

Buch | Hardcover

216 Seiten

2015 | 2nd ed. 2015
Humana Press Inc. (Verlag)
978-1-4939-2364-9 (ISBN)

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This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Fast Masking Of Repeated Primer Binding Sites In Eukaryotic Genomes.- Primer Design for PCR Reactions in Forensic Biology.- Design of Primers and Probes For Quantitative Real-Time PCR Methods.- Large-scale Nucleotide Sequence Alignment And Sequence Variability Assessment To Identify the Evolutionarily Highly Conserved Regions For Universal Screening PCR Assay Design: An Example Of Influenza A Virus.- Low-concentration Initiator Primers Improve The Amplification Of Gene Targets With High Sequence Variability.- Multiplex PCR Primer Design for Simultaneous Detection of Multiple Pathogens.- Degenerate Primer Design for Highly Variable Genomes.- Allele-specific Real Time Polymerase Chain Reaction As A Tool For Urate Transporter 1 Mutation Detection.- MultiPLX: Automatic Grouping And Evaluation Of PCR Primers.- In silico PCR Primer Designing and Validation.- Primer Design using Primer Express® for SYBR Green Based Quantitative PCR.- Designing Primers for SNaPshot Technique.- Rapid and Simple Method of qPCR Primer Design.- PRIMEGENSw3: A Web-based Tool for High-throughput Primer and Probe Design.- Selecting Specific PCR Primers with MFEprimer.

Reihe/Serie	Methods in Molecular Biology ; 1275
Zusatzinfo	45 Illustrations, color; 28 Illustrations, black and white; XII, 216 p. 73 illus., 45 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Sachbuch/Ratgeber ► Natur / Technik ► Naturführer
	Informatik ► Weitere Themen ► Bioinformatik
	Medizin / Pharmazie ► Medizinische Fachgebiete
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Schlagworte	degenerate PCR • DNA amplification • forensic biology • genotyping • in silico PCR primer designer • Multiplex PCR • Polymerase Chain Reaction (PCR) • Quantitative PCR
ISBN-10	1-4939-2364-1 / 1493923641
ISBN-13	978-1-4939-2364-9 / 9781493923649
Zustand	Neuware