NanoBiotechnology Protocols

Sandra J Rosenthal, David W Wright (Herausgeber)

Media-Kombination

230 Seiten

2005
Humana Press Inc.
978-1-58829-276-6 (ISBN)

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Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.

Applications of Quantum Dots in Biology.- Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots.- Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates.- Peptide-Conjugated Quantum Dots.- Quantum Dot-Encoded Beads.- Use of Nanobarcodes® Particles in Bioassays.- Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging.- Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies.- Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters.- Surface-Functionalized Nanoparticles for Controlled Drug Delivery.- Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis.- Structural DNA Nanotechnology.- Nanostructured DNA Templates.- Probing DNA Structure With Nanoparticles.- Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells.- Real-Time Cell Dynamics With a Multianalyte Physiometer.

Erscheint lt. Verlag	27.4.2005
Reihe/Serie	Methods in Molecular Biology ; 303
Zusatzinfo	XII, 230 p. With CD-ROM.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	1-58829-276-2 / 1588292762
ISBN-13	978-1-58829-276-6 / 9781588292766
Zustand	Neuware