Nanopore Technology

Methods and Protocols

Buch | Hardcover

231 Seiten

2020 | 1st ed. 2021
Springer-Verlag New York Inc.
978-1-0716-0805-0 (ISBN)

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This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.

Preparation of Fragaceatoxin C (FraC) Nanopores.- Preparation of Cytolysin A (ClyA) Nanopores.- Building Synthetic Transmembrane Peptide Pores.- Design and Assembly of Membrane-Spanning DNA Nanopores.- Determining the Orientation of Porins in Planar Lipid Bilayers.- Revelation of Function and Inhibition of Wza through Single Channel Studies.- Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore.- Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules.- A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore.- Oligonucleotide-Directed Protein Threading through a Rigid Nanopore.- Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP.- Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel.- Free Energy Minimization for Vesicle Translocation through a Narrow Pore.- Single Ion-Channel Analysis in Droplet Interface Bilayer.- Continuous and Rapid Solution Exchangein a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer.- Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.

Erscheinungsdatum	21.09.2020
Reihe/Serie	Methods in Molecular Biology ; 2186
Zusatzinfo	43 Illustrations, color; 14 Illustrations, black and white; X, 231 p. 57 illus., 43 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
	Technik ► Umwelttechnik / Biotechnologie
ISBN-10	1-0716-0805-3 / 1071608053
ISBN-13	978-1-0716-0805-0 / 9781071608050
Zustand	Neuware