Analysis of Nucleic Acids by Capillary Electrophoresis

Buch | Softcover

X, 314 Seiten

2013 | 1. Softcover reprint of the original 1st ed. 1997
Vieweg & Teubner (Verlag)
978-3-322-91017-2 (ISBN)

Artikel merken

During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe cialization is unavoidable. This evolution is also reflected in the development of instrumen tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.

Dr. Christoph Heller ist Wissenschaftler am Max-Planck-Institut für molekulare Genetik in Berlin. The editor, Dr. Christoph Heller, is currently working at the Max-Planck-Institute for Molecular Genetics in Berlin-Dahlem, Germany. Since 1985 he is working in the field of DNA separation, both in conventional gels and - since five years - also in capillaries.

I Basic Concepts.- 1 The Separation Matrix.- 2 Electrophoresis Theories.- 3 Microscopic Studies on the Migration Mechanism.- II Factors Affecting the Separation.- 4 Influence of Polymer Concentration and Electric Field.- 5 Effects of Sample Matrix and Injection.- 6 Developments in Capillary Coating and DNA Separation Matrices.- III Applications.- 7 Analysis of Oligonucleotides and Antisense DNA Analogs.- 8 Separation of DNA Restriction Fragments and PCR Products.- 9 Pulsed-Field Capillary Electrophoresis of Large DNA.- 10 Capillary Electrophoresis for DNA Sequencing.- 11 Mutational Analysis with Capillary Electrophoresis.- 12 Analysis of Transfer RNA and 5S Ribosomal RNA.- IV Recent Developments.- 13 Update on Improvements in DNA Separation.

Erscheint lt. Verlag	6.1.2013
Reihe/Serie	CHROMATOGRAPHIA CE-Series
Mitarbeit	Herausgeber (Serie): Kevin D. Altria
Zusatzinfo	X, 314 p.
Verlagsort	Wiesbaden
Sprache	englisch
Maße	148 x 210 mm
Gewicht	430 g
Themenwelt	Technik
Schlagworte	Chemistry • Development • Elektrophorese • Technology
ISBN-10	3-322-91017-2 / 3322910172
ISBN-13	978-3-322-91017-2 / 9783322910172
Zustand	Neuware