A Laboratory Guide to Genomic Sequencing
Springer Basel (Verlag)
978-3-7643-1925-0 (ISBN)
I. Introduction.- A Safety Considerations.- B Introduction.- C The Principle of Genomic Sequencing.- II Theoretical Background.- A Basic Theoiy of Genomic Sequencing.- B Flow Diagram.- III Experimental.- 1 Isolation of Genomic DNA.- 2 Restriction Digest of Genomic DNA.- 3 Chemical Sequencing Reactions on Restricted DNA.- 4 Separation of Reaction Products on a Sequencing Gel.- 5 Electrotransfer to Nylon Membranes.- 6 Immobilization of DNA on a Nylon Membrane.- 7 Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes.- 8 Processing of the Hybridized Filters.- 9 Autoradiography and Photography.- 10 Cloning of DNA Probe in M13.- 11 Large-Scale Preparation of Cloned DNA in M13.- 12 Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes.- 13 Purification of Labeled Single-Stranded Probes.- IV Trouble-Shooting Guide and Examples.- V Appendix.- VI Bibliography.
| Erscheint lt. Verlag | 1.1.1987 |
|---|---|
| Reihe/Serie | Biomethods |
| Zusatzinfo | 164 p. 31 illus. |
| Verlagsort | Basel |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Gewicht | 475 g |
| Themenwelt | Geisteswissenschaften |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Sozialwissenschaften | |
| Schlagworte | chromosome • DNA • Gene • Membrane |
| ISBN-10 | 3-7643-1925-9 / 3764319259 |
| ISBN-13 | 978-3-7643-1925-0 / 9783764319250 |
| Zustand | Neuware |
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