Advancing Methods for Biomolecular Crystallography

Randy Read, Alexandre G. Urzhumtsev, Vladimir Y. Lunin (Herausgeber)

500 Seiten

1., 2013
Springer Netherland (Hersteller)
978-94-007-6236-7 (ISBN)

Infos

This work presents a snapshot of the state of the art of modern biomolecular crystallography, from crystallisation through structure determination and even interactive presentation on the web.  Methods driving the latest automated structure determination pipelines are explained, as well as how to deal with problems such as crystal pathologies that still demand expert analysis.  These methods are illustrated through their application to problems of great biological interest, such as the molecular machinery underlying the complement pathway, the mechanism of action of monoamine oxidase inhibitors, and the structure of the eukaryotic ribosome.  Complementary approaches, such as neutron diffraction, small angle X-ray scattering, coherent diffraction and computational modelling, are also explored.

Inhalt

The Zen of Model Anomalies – Correct most of them. Treasure the meaningful valid few. Live serenely with the rest!; J. S. Richardson and D. C. Richardson.- Total Chemical Protein Synthesis for the Determination of Novel X-Ray Structures by Racemic Protein Crystallography; K. Mandal and S.B.H. Kent.- Crystal Pathologies; T. O. Yeates.- Crystallizing Membrane Proteins for Structure-Function Studies Using Lipidic Mesophases; M. Caffrey.- Searching for needles in haystacks: Automation and the task of Crystal Structure determination; S. McSweeney.- Data processing: how good are my data really?; K. Diederichs and P. A. Karplus.- Radiation Damage in Macromolecular Crystallography: what is it and why do we care?; E. F. Garman.- Elemental analysis of proteins by Proton Induced X-ray Emission (microPIXE); E. F. Garman, O. B. Zeldin.- X-rays-induced cooperative atomic movement in a protein crystal; T. Petrova et al.- Everything happens at once – Deconvolving systematic effects in X-ray data processing; D. Borek and Z. Otwinowski.- Extending the reach of molecular replacement; R.J. Read et al.- Phasing through location of small fragments and density modification with ARCIMBOLDO; I. Usón et al.- SAD/MAD phasing; Z. Dauter.- Macromolecular Phasing: Solving the Substructure; T. Gru¨ne.- Advanced Applications of Shelxd and Shelxe; T.Gru¨ne.-  Use of a weak anomalous signal for phasing in protein crystallography: Reflection from personal experience;  F. Frolow.- Ab initio low resolution phasing; V. Y. Lunin et al.- Model-building and Reduction of Model Bias in Electron Density Maps; T. C. Terwilliger.- Using Coot to Model Protein and Ligand Structures using X-ray data; P. Emsley.- Crystallographic Structure Refinement in a Nutshell; P. V. Afonine and P. D. Adams.- Crystallographic maps and models at low and at subatomic resolutions; A. Urzhumtsev et al.- Recent Advances in Low Resolution Refinement Tools in REFMAC; R. A. Nicholls et al.- High resolution macromolecular crystallography; M. Jaskolski.- Publishing in Proteopedia: The Guide; J. Prilusky et al.- Proteolysis, complex formation and conformational changes drive the complement pathways; P. Gros and F. Forneris.- Monoamine Oxidase Inhibitors: Diverse and Surprising Chemistry with expanding Pharmacological Potential; C. Binda et al.- Structure of the eukaryotic ribosome: tips and tricks; S. Melnikov.- Neutron Protein Crystallography. How to proceed the experiments to obtain the structural information of hydrogen, protons and hydration in Bio-macromolecules; N. Niimura.- Coherent diffraction and holographic imaging of individual biomolecules using low-energy electrons; T. Latychevskaia et al.-  Structure analysis of biological macromolecules by small-angle X-ray scattering; D. I.Svergun.- Protein structure modeling with Rosetta: Case studies in structure prediction and enzyme repurposing; F. DiMaio.

Produkt-Details

• Reihe/Serie: NATO Science for Peace and Security Series
• Sprache: englisch
• Schlagworte: Automated structural biology • Erice crystallography course • Protein Crystallography
• ISBN-10: 94-007-6236-4 / 9400762364
• ISBN-13: 978-94-007-6236-7 / 9789400762367
• Zustand: Neuware