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Macromolecular Crystallography Protocols, Volume 2

Structure Determination

Sylvie Doublie (Herausgeber)

Buch | Hardcover

284 Seiten

2007
Humana Press Inc. (Verlag)
978-1-58829-902-4 (ISBN)

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In the decade since publication of the first edition of Crystallographic Methods and Protocols the field has seen several major developments that have both accelerated the pace of structure determination and made crystallography accessible to a broader range of investigators. Volume I, Preparation and Crystallization of Macromolecules is dedicated to the crystallization and ways to increase the odds of obtaining crystals in macromolecules, while Volume 2, Structure Determination, covers both computational methods for characterizing crystals and solving structures.

Cryocrystallography of Macromolecules.- Determination of Reaction Intermediate Structures in Heme Proteins.- Annealing Macromolecular Crystals.- First Analysis of Macromolecular Crystals.- X-Ray Data Collection From Macromolecular Crystals.- Characterizing a Crystal From an Initial Native Dataset.- Molecular Replacement.- Phase Determination Using Halide Ions.- The Same But Different.- Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement With Anomalous Scattering Data Using Shake-and-Bake.- Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments.- Automated Structure Solution With autoSHARP.- to Macromolecular Refinement.- Quality Control and Validation.- Crystallographic Software.

Reihe/Serie	Methods in Molecular Biology ; 364
Zusatzinfo	70 Illustrations, black and white; XII, 284 p. 70 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	155 x 235 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-58829-902-3 / 1588299023
ISBN-13	978-1-58829-902-4 / 9781588299024
Zustand	Neuware