CCN Proteins
Springer-Verlag New York Inc.
978-1-0716-2746-4 (ISBN)
Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.
CCN Proteins - Cellular Communication Network Factors: Expanding their Repertoire toward a New Concept.- Optimal CCN4 Immunofluorescence for Tissue Microarray.- Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes.- Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and Cells.- Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes.- Evaluation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance.- Protocols for Screening Peptides Binding to CCN Family Proteins and their Extended Utility.- Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism.- Analyses of the Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression.- Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network.- Utilizing Public Molecular Biological Databases for CCN Family Research.- Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation.- Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy.- The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues.- Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis.- Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins.- Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells.- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo.- Angiogenesis Assays for the Analysis of CCN Proteins.- Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis.- Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1.- Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models.- Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins.- Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products.- Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain and Loss of Function Approaches.- Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney.- Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.
| Erscheinungsdatum | 13.11.2023 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology |
| Zusatzinfo | 66 Illustrations, color; 20 Illustrations, black and white; XIV, 430 p. 86 illus., 66 illus. in color. |
| Verlagsort | New York, NY |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Naturwissenschaften ► Chemie | |
| Schlagworte | Cellular Communication Network • extracellular vesicles • ILISA • posttranscriptional regulation • western blottin |
| ISBN-10 | 1-0716-2746-5 / 1071627465 |
| ISBN-13 | 978-1-0716-2746-4 / 9781071627464 |
| Zustand | Neuware |
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