Embryonic Stem Cell Protocols

Buch | Softcover

363 Seiten

2023 | 4th ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2439-5 (ISBN)

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This fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.

lt;p>1. Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens

Oriana Genolet, Liat Ravid Lustig, and Edda G. Schulz

2. Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions

Taha Bartu Hayal and Aysegül Dogan

3. Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish

Fatima Bianca A. Dessouki, Pawan K. Singal, and Dinender K. Singla

4. Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells

Federico Pecori and Shoko Nishihara

5. High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model

Olga Gordeeva

6. Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications

Loriana Vitillo and Ludovic Vallier

7. Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol

Hatice Burcu Sisli, Selinay Senkal, Derya Sagraç, Taha Bartu Hayal, and Aysegül Dogan

8. ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells

Divya Desai and Prasad Pethe

9. Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids

Yoshitomo Ueda, Stephen T. Moore, and Eri Hashino

10. Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs)

Bibiana Correia, Maria Inês Sousa, and João Ramalho-Santos

11. Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Arzuhan Koc and Esra Cagavi

12. Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo

Katsuhiro Konno, Jingjing Kobayashi-Sun, Fumio Arai, Isao Kobayashi, and Daisuke Sugiyama

13. Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells

Wen Huang, Sze Wan Fong, William Shu Biu Yeung, and Yin-Lau Lee

14. Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions

Luciana Isaja, Sofía Luján Ferriol-Laffouillere, Sofía Mucci, María Soledad Rodríguez-Varela, and Leonardo Romorini

15. A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells

Helena Covelo-Molares, Yara Souto, Miguel Fidalgo, and Diana Guallar

16. Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation

Magdalena Guerra-Crespo, Omar Collazo-Navarrete, Rodrigo Ramos-Acevedo, Carmen Alejandra Morato-Torres, and Birgitt Schüle

17. A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation

Jianqun Zheng, Ying Ye, Qiushi Xu, Wei Xu, Wensheng Zhang, and Xi Chen

18. Osteogenic Differentiation from Mouse Embryonic Stem Cells

Zahra Alvandi and Michal Opas

19. Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation

Manar Elkenani and Belal A. Mohamed

20. Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions

Emre Balbasi, Gozde Guven, and Nihal Terzi Çizmecioglu

21. Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages

Emre Balbasi, Dersu Sezginmert, Ceren Alganatay, and Nihal Terzi Çizmecioglu

22. Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling

Rubens Gomes-Júnior, Patrícia Shigunov, Bruno Dallagiovanna, and Isabela Tiemy Pereira

23. Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors

Giulia Pavani, Joshua Klein, Deborah L. French, and Paul Gadue

24. Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS

Jirina Tyleckova, Jakub Cervenka, Ievgeniia Poliakh, Jaromir Novak, Katerina Vodickova Kepkova, Helena Kupcova Skalnikova, and Petr Vodicka

Erscheinungsdatum	29.07.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	1 Illustrations, black and white; XIII, 363 p. 1 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	cell lineage • ESCs • induced pluripotent stem cells • Organoids • Stem Cells
ISBN-10	1-0716-2439-3 / 1071624393
ISBN-13	978-1-0716-2439-5 / 9781071624395
Zustand	Neuware