Membrane Trafficking

Methods and Protocols

Buch | Softcover

407 Seiten

2023 | 1st ed. 2022
Springer-Verlag New York Inc.
978-1-0716-2211-7 (ISBN)

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This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.

lt;p>Part I: The Exocytic Pathway

1. Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System

Marine D. Camus and Stephane M. Camus

2. Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo

Julia Dancourt and Grégory Lavieu

3. Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)

Cedric S. Asensio

4. Spatial and Temporal Control of Protein Secretion with Light

Ashley M. Bourke and Matthew J. Kennedy

5. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing

Syed Mukhtar Ahmed

6. Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting

Kunrong Mei, Di-Ao Liu, and Wei Guo

7. Imaging Insulin Granule Dynamics in Human Pancreatic beta-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy

Fei Kang and Herbert Y. Gaisano

8. Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique

Jihong Gong, Xiaofei Yang, and Cong Ma

9. Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons

Tong Wang and Frédéric A. Meunier

10. Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse

Tamara Basta, Garry P. Morgan, Eileen T. O'Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H.B. Stowell

11. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion

Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu

12. Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)

Yingke Xu, Luhong Jin, and Derek Toomre

Part II: The Endocytic Pathway

13. Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy

Bijeta Prasai and Justin W. Taraska

14. Endocytosis Assays Using Cleavable Fluorescent Dyes

Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen

15. Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2

Bing Wang, Rui Yang, Yuan Tian, and Qian Yin

16. Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches

Amulya Priya and Sunando Datta

17. Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches

Priyanka Sharma, Sameena Parveen, and Sunando Datta

18. Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins

Marijn G.J. Ford and Rajesh Ramachandran

19. Measurement of Lysosome Positioning by Shell Analysis and Line Scan

Chad D. Williamson, Carlos M. Guardia, Raffaella De Pace, Juan S. Bonifacino, and Amra Saric

Part III: Emerging Topics in Membrane Trafficking

20. Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT)

Bobae Hyeon, Mai Khanh Nguyen, and Won Do Heo

21. Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways

Daniel R. Gulbranson

22. Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons

Juhyung Lee and Yihong Ye

23. Single-Molecule Optical Tweezers Study of Protein-Membrane Interactions

Lu Ma, Jinghua Ge, Xin Bian, and Yongli Zhang

24. Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes

Max Piffoux, Amanda Karine Andriola Silva, Florence Gazeau, and David Tareste

25. Quantitative Measurement of Extracellular Vesicle Content Delivery within Acceptor Cells

Émeline Bonsergent, Shéryl Bui, and Grégory Lavieu

Erscheinungsdatum	18.07.2023
Reihe/Serie	Methods in Molecular Biology
Zusatzinfo	82 Illustrations, color; 9 Illustrations, black and white; XIV, 407 p. 91 illus., 82 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	CRISPR genetic screens • endocytic pathway • Endomembrane systems • exocytic pathway • Membrane Biology
ISBN-10	1-0716-2211-0 / 1071622110
ISBN-13	978-1-0716-2211-7 / 9781071622117
Zustand	Neuware