Chemokine-Glycosaminoglycan Interactions

Methods and Protocols

Buch | Hardcover

262 Seiten

2022 | 1st ed. 2023
Springer-Verlag New York Inc.
978-1-0716-2834-8 (ISBN)

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This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy.- Remote Ischemic Pre-Conditioning (RIPC).- Mouse Corneal Transplantation.- Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant.- Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade.- Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion.- In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor.- The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.- Flow Cytometry Analysis of Immune Cell Responses.- Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence.- Protein Microarrays and Their Fabrication.- NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions.- Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions.- Isolation and Compositional Analysis of Glycosaminoglycans.- How to Design Peptides.- Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins.- Producing Biologics with Defined N-Glycosylation in Plants.- Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery.

Erscheinungsdatum	17.11.2022
Reihe/Serie	Methods in Molecular Biology ; 2597
Zusatzinfo	1 Illustrations, black and white; XV, 262 p. 1 illus.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
Themenwelt	Naturwissenschaften ► Biologie ► Zellbiologie
Schlagworte	Autoimmune disorders • GAG networks • Immune Cell Trafficking • Immune responses • Organ and tissue models • polysaccharides
ISBN-10	1-0716-2834-8 / 1071628348
ISBN-13	978-1-0716-2834-8 / 9781071628348
Zustand	Neuware