Mitochondrial Regulation

Methods and Protocols

Carlos M. Palmeira, Anabela P. Rolo (Herausgeber)

Buch | Hardcover

318 Seiten

2021 | 2nd ed. 2021
Springer-Verlag New York Inc.
978-1-0716-1432-7 (ISBN)

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This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.

Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.- Determination of Oxidative Phosphorylation Complexes Activities.- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function.- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs).- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes.- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies.- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases.- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria.- Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry.- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD).- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria.- Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis.- Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content.- Following the Dynamism of the Mitochondrial Network in T Cells.- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.

Erscheinungsdatum	11.06.2021
Reihe/Serie	Methods in Molecular Biology ; 2310
Zusatzinfo	47 Illustrations, color; 17 Illustrations, black and white; XII, 318 p. 64 illus., 47 illus. in color.
Verlagsort	New York, NY
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
	Naturwissenschaften ► Physik / Astronomie ► Angewandte Physik
Schlagworte	cellular metabolism • Dynamic Properties • mitochondria • mitochondrial function • Signaling events
ISBN-10	1-0716-1432-0 / 1071614320
ISBN-13	978-1-0716-1432-7 / 9781071614327
Zustand	Neuware