In Situ Hybridization

Principles and Practice

Julia M. Polak, James O'D. McGee (Herausgeber)

Buch | Hardcover

224 Seiten

1999 | 2nd Revised edition
Oxford University Press (Verlag)
978-0-19-854880-5 (ISBN)

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The technique of in situ hybridisation is vital to molecular biologists and their understanding of the pathophysiology of cellular functions. This practical guide covers all aspects of in situ hybridisation, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology.

1. Principles of in situ hybridization ; 2. The design and generation of probes for in situ hybridization ; 3. The preparation of non-radioisotopic hybridization probes ; 4. Principles and applications of complementary RNA probes ; 5. Quantification of in situ hybridization ; 6. Quantification of grains in in situ hybridization ; 7. Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization ; 8. Single and simultaneous nucleic acid detection in clinical samples ; 9. Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels ; 10. Super sensitive in situ DNA and RNA localisation

Erscheint lt. Verlag	28.1.1999
Zusatzinfo	16 colour plates, 17 black and white photographs and 33 line figures
Verlagsort	Oxford
Sprache	englisch
Maße	159 x 240 mm
Gewicht	507 g
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Laboratoriumsmedizin
	Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	0-19-854880-X / 019854880X
ISBN-13	978-0-19-854880-5 / 9780198548805
Zustand	Neuware