Oogenesis
Humana Press Inc. (Verlag)
978-1-4939-8141-0 (ISBN)
oogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guides
readers through protocols on models of
developmental biology, oogenesis in plants, worms, fruit flies,
mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and cutting-edge, Oogenesis:
Methods and Protocols aims to ensure successful results in the further
study of this vital field.
Histological Analysis of the Arabidopsis
Gynoecium and Ovules Using Chloral Hydrate Clearing and Differential Interference
Contrast Light Microscopy.- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans.- Antibody Staining In Drosophila Germaria.- In Vitro<
Culturing and Live Imaging of Drosophila
Egg Chambers: A History and Adaptable Method.- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cells
in Drosophila Melanogaster.- Analysis of Phagocytosis in the Drosophila Ovary.- Immuno-Gold Labeling Of Drosophila Follicles
For Transmission Electron Microscopy.- Preparation of Drosophila Follicles for
Transmission Electron Microscopy.- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling,
Annotating, and Analysing your Data.- Live Imaging of
Centriole Dynamics by Fluorescently-Tagged Proteins un Starfish Oocyte Meiosis.- High-Pressure Freezing Electron Microscopy of
Zebrafish Oocytes.- Cytoskeleton and Cytoskeleton-Bound RNA Visualization
in Frog and Insect Oocytes.- RNA FISH, DNA FISH and Chromosome Painting of
Chicken Oocytes.- Preparation of Cell Lysate from Mouse Oocytes for Western Blotting Analysis.- Quantitative Microinjection of Morpholino Antisense Oligonucleotides into Mouse
Oocytes to Examine Gene Function in Meiosis-I.- Monitoring Calcium Oscillations in Fertilized
Mouse Eggs.- Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse
Oocytes.- Isolation of Mammalian Oogonial Stem Cells by Antibody-Based Fluorescence
Activated Cell Sorting.- Immunohistochemistry of Paraffin Sections from Mouse Ovaries.
| Erscheinungsdatum | 18.08.2018 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1457 |
| Zusatzinfo | 50 Illustrations, black and white; X, 277 p. 50 illus. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Chicken • Mosquitos • Mouse • Oogenesis • plants |
| ISBN-10 | 1-4939-8141-2 / 1493981412 |
| ISBN-13 | 978-1-4939-8141-0 / 9781493981410 |
| Zustand | Neuware |
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