Construction of plasmid-based expression vector for Bacillus subtilis

In cytoplasm, on the surface and as secretion into culture medium

Hoang D. Nguyen (Autor)

Buch | Softcover

136 Seiten

2017
Scholar's Press (Verlag)
978-620-2-30072-8 (ISBN)

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Plasmids are useful tools to study gene expression and their function. However, most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental system to immobilize proteins on the cell wall of B. subtilis. The sorting of surface proteins to the cell wall in B. subtilis has been investigated aiming to anchor recombinant protein on the surface.

Hoang D. Nguyen obtained his Ph.D. certificate at the University of Bayreuth, Germany. He is now an associate professor of Microbiology at the University of Science, Vietnam National University-Hochiminh City. He has a keen interest in the development of expression systems for Bacillus subtilis and production of recombinant proteins.

Erscheinungsdatum	16.09.2021
Sprache	englisch
Maße	150 x 220 mm
Gewicht	221 g
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
Themenwelt	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Bacillus subtilis • gene expression • Pgrac • pHCMC • Protein production • recombinant protein • secretion • sortase • sorting sequence • surface display
ISBN-10	620-2-30072-8 / 6202300728
ISBN-13	978-620-2-30072-8 / 9786202300728
Zustand	Neuware