Crystallization of Nucleic Acids and Proteins: A Practical Approach
Oxford University Press (Verlag)
978-0-19-963246-6 (ISBN)
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In the last decade crystallography has emerged as a key tool for understanding the structures of biological macromolecules, yet the crystallization techniques themselves have been perceived as difficult to perform. This book provides the first detailed and rational guide to producing crystals of proteins and nucleic acids for diffraction studies. Standard crystallization methods are given, along with procedures for the preparation and handling of macromolecules.
Detailed laboratory protocols and the underlying theoretical background are brought together, providing researchers with the conceptual and methodological tools necessary to exploit these powerful techniques.
R. Giegé & A. Ducruix: An introduction to the crystallogenesis of biological macromolecules; B. Lorber & R. Giegé: Preparation and handling of biological macromolecules for crystallization; C.W. Carter: Design of crystallization experiments and protocols; A. Ducruix & R. Giegé: Methods of crystallization; E. Stura & I.A. Wilson: Seeding techniques; M.C. Robert, K. Provost, & F. Lefaucheux: Crystallization in gels and related
methods; A.C. Dock-Bregeon & D. Moras: Crystallization of nucleic acids and co-crystallization of proteins and nucleic acids; F. Reiss-Husson: Crystallization of membrane proteins; M. Ries-Kaut & A. Ducruix: Phase diagrams; V. Mikol & R. Giegé: The physical chemistry of protein crystallization; E.A. Stura & P. Chen: Soaking of crystals; L.
Sawyer & M.A. Turner: X-ray analysis; K.B. Ward, M.A. Perozzo & W.M. Zuk: Automating crystallization experiments; S. Doublie & C.W. Carter: Preparation of selenomethionyl protein crystals; Appendix; Index.
| Erscheint lt. Verlag | 27.2.1992 |
|---|---|
| Reihe/Serie | Practical Approach Series ; 86 |
| Zusatzinfo | halftones, line illustrations, tables |
| Verlagsort | Oxford |
| Sprache | englisch |
| Maße | 155 x 234 mm |
| Gewicht | 548 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Geowissenschaften ► Mineralogie / Paläontologie | |
| ISBN-10 | 0-19-963246-4 / 0199632464 |
| ISBN-13 | 978-0-19-963246-6 / 9780199632466 |
| Zustand | Neuware |
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