Programmed Cell Death
Humana Press Inc. (Verlag)
978-1-4939-3579-6 (ISBN)
This volume contains
protocols specifically designed for studying programmed cell death, and also
discusses recent advances in techniques that span broader areas of biology that
have been recently used or that have potential to be incorporated into cell death
research. The protocols are mostly described in the context of mammalian
systems, but also cover other systems such as plants, Drosophila, and
yeast. Programmed Cell Death: Methods and Protocols is comprised of 20
chapters: Chapters 1-5 describe apoptosis detection techniques; Chapter 6-9
describe methods for studying apoptosis associated with various pathologies in
different organs including the lymphoid compartment, intestinal epithelium,
granulocytes, and cardiomyocytes; Chapter 11-13 cover protocols and techniques
for studying apoptosis in non-mammalian systems; Chapters 14-16 cover
biochemical and biophysical methods for studying Bcl-2 family protein dynamics
and protein-protein interactions during apoptosis;and the last four chapters
explore protocols that are useful not only in apoptosis research but in wider
areas of biological research, such as genome editing, inducible transgenes, and
proteomics. Written in the highly successful Methods in Molecular Biology
aeries format, chapters include introductions to their respective topics, lists
of the necessary material and reagents, step-by-step, readily reproducible
laboratory protocol, and tips on troubleshooting and avoiding known pitfalls.
Thorough
and cutting-edge, Programmed Cell Death: Methods and Protocols is a
comprehensive and valuable resource for researchers, ranging from beginner to
expert, in their studies on programmed cell death.
Detection of Apoptotic Versus Autophagic Cell Death by Flow Cytometry.- In Vivo Apoptosis Imaging Using
Site-Specifically 68Ga-Labeled Annexin V.- Detection of Active Caspases
during Apoptosis Using Fluorescent Activity-Based Probes.- Detection of Initiator Caspase
Induced Proximity in Single Cells by Caspase Bimolecular Fluorescence Complementation.- In Vitro Use of Peptide Based Substrates
and Inhibitors of Apoptotic Caspases.- Experimental In Vivo Sepsis Models
to Monitor Immune Cell Apoptosis and Survival in Laboratory Mice.- Analysis of Cell Death Induction
in Intestinal Organoids In Vitro.- In Vitro Differentiation of Mouse
Granulocytes.- Hydrodynamic Injection as a Method
of Gene Delivery in Mice: A Model of Chronic Hepatitis B Virus Infection.- Isolation of Cardiomyocytes
and Cardiofibroblasts for Ex-Vivo Analysis.- Detection of Cell Death in Drosophila Tissues.- Methods to Study Plant Programmed
Cell Death.- Modeling Metazoan Apoptotic Pathways
in Yeast.- Characterizing Bcl-2 Family Protein
Conformation and Oligomerization Using Cross-Linking and Antibody Gel-Shift in Conjunction
with Native PAGE.- Using Förster-Resonance Energy
Transfer to Measure Protein Interactions between Bcl-2 Family Proteins on Mitochondrial
Membranes.- Preparing Samples for Crystallization
of Bcl-2 Family Complexes.- Screening Strategies for
TALEN Mediated Gene Disruption.- Using CRISPR/Cas9 Technology
for Manipulating Cell Death Regulators.- Lentiviral Vectors to Analyze
Cell Death Regulators.- Proteomic Profiling of Cell Death:
Stable Isotope Labeling and Mass Spectrometry Analysis.
| Erscheinungsdatum | 08.04.2016 |
|---|---|
| Reihe/Serie | Methods in Molecular Biology ; 1419 |
| Zusatzinfo | 39 Illustrations, color; 20 Illustrations, black and white; XI, 291 p. 59 illus., 39 illus. in color. With online files/update. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | Apoptosis • apoptosis detection techniques • autophagic cell death processes • Bcl-2 family protein dynamics • caspase activity initiation at single cell level • delineating the molecular mechanisms • Drosophila • Genome editing • metazoan apoptosis process • peptide-based techniques for detecting caspases • protein-protein interactions |
| ISBN-10 | 1-4939-3579-8 / 1493935798 |
| ISBN-13 | 978-1-4939-3579-6 / 9781493935796 |
| Zustand | Neuware |
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