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Nonribosomal Peptide and Polyketide Biosynthesis

Methods and Protocols

Bradley Evans (Herausgeber)

Buch | Hardcover

281 Seiten

2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3373-0 (ISBN)

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This volume presents an overview of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) structure and function. It then continues with methods for the analysis of these pathways including conventional enzymological assays, contemporary mass spectrometric analysis techniques, specialized molecular biological approaches applicable to NRPSs and PKSs, and small molecule analysis tools tailored to this very special class of natural products, and concludes by examining bioinformatics tools for the analysis of these enzymes, pathways, and molecules. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nonribosomal Peptide and Polyketide Biosynthesis: Methods and Protocols serves as a valuable reference for those experienced in studying NRPS and PKS enzymes, pathways, and natural products as well as a gateway for those just entering the field.

Structural Biology of Nonribosomal Peptide Synthetases.- The Assembly Line Enzymology of Polyketide Biosynthesis.- Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay.- Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains.- Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases.- Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis.- Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro.- The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis.- Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC—MS/MS-Based Proteomics.- Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi.- In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.- Secondary Metabolic Pathway-Targeted Metabolomics.- Annotating and Interpreting Linear and Cyclic
Peptide Tandem Mass Spectra.- Bioinformatics Tools for the Discovery of New
Nonribosomal Peptides.- The Use of ClusterMine360 for the Analysis of
Polyketide and Nonribosomal Peptide Biosynthetic Pathways.- Alignment-Free
Methods for the Detection and Specificity Prediction of Adenylation Domains.- Characterization
of Nonribosomal Peptide Synthetases with NRPSsp.

Erscheinungsdatum	12.12.2015
Reihe/Serie	Methods in Molecular Biology ; 1401
Zusatzinfo	49 Illustrations, color; 31 Illustrations, black and white; XI, 281 p. 80 illus., 49 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Naturwissenschaften ► Biologie ► Allgemeines / Lexika
	Naturwissenschaften ► Biologie ► Biochemie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	Bioinformatics • Biosynthetic enzymes • Enzymological assays • Mass Spectrometry • Nonribosomal peptide synthetases (NRPSs) • Polyketide synthases (PKSs) • Small molecule analysis
ISBN-10	1-4939-3373-6 / 1493933736
ISBN-13	978-1-4939-3373-0 / 9781493933730
Zustand	Neuware