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Poliovirus

Methods and Protocols

Javier Martín (Herausgeber)

Buch | Hardcover

341 Seiten

2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3291-7 (ISBN)

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This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication.- Poliovirus Laboratory Based Surveillance: An Overview.- Isolation and Characterisation of Enteroviruses from Clinical Samples.- Isolation and Characterisation of Poliovirus in Cell Culture Systems.- Molecular Characterization of Polio from Environmental Samples – ISSP, The Israeli Sewage Surveillance Protocol.- Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays.- A Transgenic Mouse Model of Poliomyelitis.- Standardized Methods for Detection of Poliovirus Antibodies.- Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis,Typing by PCR and Real-time RT-PCR.- Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative.- Phylogenetic Analysis of Poliovirus Sequences.- Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA.- A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses.- Methods to Monitor Molecular Consistency of Oral Polio Vaccine.- Methods for the Quality Control of Inactivated Poliovirus Vaccines.- Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays.- Identification and Analysis of Antiviral Compounds against Poliovirus.

Erscheint lt. Verlag	17.3.2016
Reihe/Serie	Methods in Molecular Biology ; 1387
Zusatzinfo	58 Illustrations, color; 17 Illustrations, black and white; XI, 341 p. 75 illus., 58 illus. in color.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	178 x 254 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Studium ► Querschnittsbereiche ► Infektiologie / Immunologie
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
Schlagworte	characterization of polioviruses • clinical laboratories • Identification • Isolation • research laboratories • RNA replication • Translation • viral antigenicity • Virus • virus cell entry
ISBN-10	1-4939-3291-8 / 1493932918
ISBN-13	978-1-4939-3291-7 / 9781493932917
Zustand	Neuware