Yeast Cytokinesis
Humana Press Inc. (Verlag)
978-1-4939-3144-6 (ISBN)
Authoritative and practical, Yeast Cytokinesis: Methods and Protocols provides practical and step-by-step detailed protocols useful for a wide audience ranging from experienced researchers to beginners in the use of yeasts.
Time-lapse Fluorescence Microscopy Of Budding Yeast Cells.- Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells.- Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division.- High-Speed Super Resolution Imaging of Live Fission Yeast Cells.- Monitoring Chitin Deposition during Septum Assembly in Budding Yeast.- Imaging Septum Formation by Fluorescence Microscopy.- Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy.- Visualization of Fission Yeast Cells By Transmission Electron Microscopy.- Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography.- Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe.- Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast.- In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins.- Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins.- Analysis of Three-Dimensional Structures of Exocyst Components.- Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis.- Detection of Phosphorylation Status of Cytokinetic Components.- Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation.- Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes.- Synchronization of the Budding Yeast Saccharomyces cerevisiae.- Fission Yeast Cell Cycle Synchronization Methods.- A Review of Fluorescent Proteins for Use in Yeast.- Visualisation and Image Analysis of Yeast Cells.- Toolbox for Protein Structure Prediction.- From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis.
| Reihe/Serie | Methods in Molecular Biology ; 1369 |
|---|---|
| Zusatzinfo | 52 Illustrations, color; 31 Illustrations, black and white; XI, 395 p. 83 illus., 52 illus. in color. |
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 178 x 254 mm |
| Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
| Naturwissenschaften ► Biologie ► Zellbiologie | |
| Schlagworte | actomyosin ring • exocyst components • F-BAR proteins • fluorescence proteins • myosin-II motor activity • protein tertiary structure • suppressor genes • transmision electron microscopy • yeast • Yeast cells |
| ISBN-10 | 1-4939-3144-X / 149393144X |
| ISBN-13 | 978-1-4939-3144-6 / 9781493931446 |
| Zustand | Neuware |
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